Applying Label-Free Quantitation to Top Down Proteomics

With the prospect of resolving whole protein molecules into their myriad proteoforms on a proteomic scale, the question of their quantitative analysis in discovery mode comes to the fore. Here, we demonstrate a robust pipeline for the identification and stringent scoring of abundance changes of whol...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2014-05, Vol.86 (10), p.4961-4968
Main Authors: Ntai, Ioanna, Kim, Kyunggon, Fellers, Ryan T, Skinner, Owen S, Smith, Archer D, Early, Bryan P, Savaryn, John P, LeDuc, Richard D, Thomas, Paul M, Kelleher, Neil L
Format: Article
Language:English
Subjects:
Biological
Biological variation
Confidence intervals
Histone Deacetylases - analysis
Histone Deacetylases - genetics
Histones
Molecules
Mutation
Mutation - genetics
Peptides
Pipelines
Proteins
Proteins - chemistry
Proteomics
Proteomics - methods
Quantitative analysis
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Symbols
Yeast
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Summary:With the prospect of resolving whole protein molecules into their myriad proteoforms on a proteomic scale, the question of their quantitative analysis in discovery mode comes to the fore. Here, we demonstrate a robust pipeline for the identification and stringent scoring of abundance changes of whole protein forms
ISSN:0003-2700
1520-6882
DOI:10.1021/ac500395k