Manipulation of mammalian cells using a single-fiber optical microbeam

The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking,...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Biomedical Optics 2008-09, Vol.13 (5), p.054049-054047
Main Authors: Mohanty, Samarendra K, Mohanty, Khyati S, Berns, Michael W
Format: Article
Language:English
Subjects:
Animals
Beams (radiation)
Cell Separation - instrumentation
Cell Separation - methods
cellular lysis
CHO Cells
Computer-Aided Design
Cricetinae
Cricetulus
Equipment Design
Equipment Failure Analysis
Fiber optics
laser microbeam
Laser Therapy - instrumentation
Micromanipulation
Micromanipulation - instrumentation
Micromanipulation - methods
Microorganisms
Microsurgery - instrumentation
Mode locking
neuron growth
Neurons
optical fiber
Optical fibers
optical micromanipulation
optical stretcher
Optical Tweezers
Reproducibility of Results
Sensitivity and Specificity
Stretching
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At high average powers, lysis of a three-dimensionally trapped cell was accomplished.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.2983663