Evaluation of Methods for the Extraction of DNA from Drinking Water Distribution System Biofilms

• Published in: Microbes and Environments 2012, Vol.27(1), pp.9-18
• Main Authors: Hwang, Chiachi, Ling, Fangqiong, Andersen, Gary L., LeChevallier, Mark W., Liu, Wen-Tso
• Format: Article
• Language: English
• Subjects: BASIC BIOLOGICAL SCIENCES; biofilm microbial community; DNA extraction; drinking water distribution system; Regular Paper
• ISSN: 1342-6311; 1347-4405
• DOI: 10.1264/jsme2.ME11132

While drinking water biofilms have been characterized in various drinking water distribution systems (DWDS), little is known about the impact of different DNA extraction methods on the subsequent analysis of microbial communities in drinking water biofilms. Since different DNA extraction methods have been shown to affect the outcome of microbial community analysis in other environments, it is necessary to select a DNA extraction method prior to the application of molecular tools to characterize the complex microbial ecology of the DWDS. This study compared the quantity and quality of DNA yields from selected DWDS bacteria with different cell wall properties using five widely used DNA extraction methods. These were further selected and evaluated for their efficiency and reproducibility of DNA extraction from DWDS samples. Terminal restriction fragment length analysis and the 454 pyrosequencing technique were used to interpret the differences in microbial community structure and composition, respectively, from extracted DNA. Such assessments serve as a concrete step towards the determination of an optimal DNA extraction method for drinking water biofilms, which can then provide a reliable comparison of the meta-analysis results obtained in different laboratories.