Long Wavelength Monitoring of Protein Kinase Activity

A family of long wavelength protein kinase fluorescent reporters is described in which the probing wavelength is pre-programmed using readily available fluorophores. These agents can assess protein kinase activity within the optical window of tissue, as exemplified by monitoring endogenous cAMP-depe...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2014-03, Vol.53 (15), p.3975-3978
Main Authors: Oien, Nathan P., Nguyen, Luong T., Jernigan, Finith E., Priestman, Melanie A., Lawrence, David S.
Format: Article
Language:English
Online Access:Get full text
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Summary:A family of long wavelength protein kinase fluorescent reporters is described in which the probing wavelength is pre-programmed using readily available fluorophores. These agents can assess protein kinase activity within the optical window of tissue, as exemplified by monitoring endogenous cAMP-dependent protein kinase activity (1) in erythrocyte lysates and (2) in intact erythrocytes using a light-activatable reporter.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201309691