Cell-free protein synthesis: Applications come of age

Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-ef...

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Bibliographic Details
Published in:Biotechnology advances 2012-09, Vol.30 (5), p.1185-1194
Main Authors: Carlson, Erik D., Gan, Rui, Hodgman, C. Eric, Jewett, Michael C.
Format: Article
Language:English
Subjects:
Biotechnology - methods
Cell-free biology
Cell-free protein synthesis
Cell-Free System
cost effectiveness
E. coli extract
genomics
High-throughput
In vitro translation
manufacturing
Personalized medicines
Protein Biosynthesis
protein synthesis
proteins
Recombinant DNA protein production
Recombinant Proteins - biosynthesis
Recombinant Proteins - therapeutic use
Synthetic biology
translation (genetics)
virus-like particles
Wheat germ extract
yields
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Summary:Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
ISSN:0734-9750
1873-1899
DOI:10.1016/j.biotechadv.2011.09.016