RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion

Abstract In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed t...

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Bibliographic Details
Published in:Cancer letters 2014-06, Vol.348 (1), p.61-70
Main Authors: Boregowda, Rajeev K, Olabisi, Oyenike O, Abushahba, Walid, Jeong, Byeong-Seon, Haenssen, Keneshia K, Chen, Wenjin, Chekmareva, Marina, Lasfar, Ahmed, Foran, David J, Goydos, James S, Cohen-Solal, Karine A
Format: Article
Language:English
Subjects:
Bone cancer
Cell growth
Cell Line, Tumor
Cell Movement - drug effects
Cell Proliferation
Cholecalciferol (Vitamin D3)
Cholecalciferol - pharmacology
Core Binding Factor Alpha 1 Subunit - genetics
Core Binding Factor Alpha 1 Subunit - metabolism
FAK
Focal Adhesion Kinase 1 - metabolism
Gene amplification
Gene expression
Gene Expression Regulation, Neoplastic
Grants
Hematology, Oncology and Palliative Medicine
Humans
Immunohistochemistry
Kinases
Matrix Metalloproteinase 13 - genetics
Medical research
Melanoma
Melanoma - genetics
Melanoma - metabolism
Melanoma - pathology
Metastasis
Migration
Motility
Neoplasm Invasiveness
Promoter Regions, Genetic
Proteins
RNA Interference
RUNX2
Signal Transduction
Skin Neoplasms - genetics
Skin Neoplasms - metabolism
Skin Neoplasms - pathology
Tissue Array Analysis
Transcription factor
Transcription factors
Transcription, Genetic
Transfection
Up-Regulation
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Summary:Abstract In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2014.03.011