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RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion
Abstract In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed t...
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| Published in: | Cancer letters 2014-06, Vol.348 (1), p.61-70 |
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| Main Authors: | , , , , , , , , , , |
| Format: | Article |
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| cites | cdi_FETCH-LOGICAL-c546t-6f58c5c18688cb0b3738d3b17280ada0d81cb16809ab9a053867d9493064eb643 |
| container_end_page | 70 |
| container_issue | 1 |
| container_start_page | 61 |
| container_title | Cancer letters |
| container_volume | 348 |
| creator | Boregowda, Rajeev K Olabisi, Oyenike O Abushahba, Walid Jeong, Byeong-Seon Haenssen, Keneshia K Chen, Wenjin Chekmareva, Marina Lasfar, Ahmed Foran, David J Goydos, James S Cohen-Solal, Karine A |
| description | Abstract In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration. |
| doi_str_mv | 10.1016/j.canlet.2014.03.011 |
| format | article |
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We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2014.03.011</identifier><identifier>PMID: 24657655</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Bone cancer ; Cell growth ; Cell Line, Tumor ; Cell Movement - drug effects ; Cell Proliferation ; Cholecalciferol (Vitamin D3) ; Cholecalciferol - pharmacology ; Core Binding Factor Alpha 1 Subunit - genetics ; Core Binding Factor Alpha 1 Subunit - metabolism ; FAK ; Focal Adhesion Kinase 1 - metabolism ; Gene amplification ; Gene expression ; Gene Expression Regulation, Neoplastic ; Grants ; Hematology, Oncology and Palliative Medicine ; Humans ; Immunohistochemistry ; Kinases ; Matrix Metalloproteinase 13 - genetics ; Medical research ; Melanoma ; Melanoma - genetics ; Melanoma - metabolism ; Melanoma - pathology ; Metastasis ; Migration ; Motility ; Neoplasm Invasiveness ; Promoter Regions, Genetic ; Proteins ; RNA Interference ; RUNX2 ; Signal Transduction ; Skin Neoplasms - genetics ; Skin Neoplasms - metabolism ; Skin Neoplasms - pathology ; Tissue Array Analysis ; Transcription factor ; Transcription factors ; Transcription, Genetic ; Transfection ; Up-Regulation</subject><ispartof>Cancer letters, 2014-06, Vol.348 (1), p.61-70</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2014 Elsevier Ireland Ltd</rights><rights>Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Jun 28, 2014</rights><rights>2014 Elsevier Ireland Ltd. All rights reserved. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c546t-6f58c5c18688cb0b3738d3b17280ada0d81cb16809ab9a053867d9493064eb643</citedby><cites>FETCH-LOGICAL-c546t-6f58c5c18688cb0b3738d3b17280ada0d81cb16809ab9a053867d9493064eb643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24657655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boregowda, Rajeev K</creatorcontrib><creatorcontrib>Olabisi, Oyenike O</creatorcontrib><creatorcontrib>Abushahba, Walid</creatorcontrib><creatorcontrib>Jeong, Byeong-Seon</creatorcontrib><creatorcontrib>Haenssen, Keneshia K</creatorcontrib><creatorcontrib>Chen, Wenjin</creatorcontrib><creatorcontrib>Chekmareva, Marina</creatorcontrib><creatorcontrib>Lasfar, Ahmed</creatorcontrib><creatorcontrib>Foran, David J</creatorcontrib><creatorcontrib>Goydos, James S</creatorcontrib><creatorcontrib>Cohen-Solal, Karine A</creatorcontrib><title>RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Abstract In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.</description><subject>Bone cancer</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation</subject><subject>Cholecalciferol (Vitamin D3)</subject><subject>Cholecalciferol - pharmacology</subject><subject>Core Binding Factor Alpha 1 Subunit - genetics</subject><subject>Core Binding Factor Alpha 1 Subunit - metabolism</subject><subject>FAK</subject><subject>Focal Adhesion Kinase 1 - metabolism</subject><subject>Gene amplification</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Grants</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kinases</subject><subject>Matrix Metalloproteinase 13 - genetics</subject><subject>Medical research</subject><subject>Melanoma</subject><subject>Melanoma - genetics</subject><subject>Melanoma - metabolism</subject><subject>Melanoma - pathology</subject><subject>Metastasis</subject><subject>Migration</subject><subject>Motility</subject><subject>Neoplasm Invasiveness</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>RNA Interference</subject><subject>RUNX2</subject><subject>Signal Transduction</subject><subject>Skin Neoplasms - genetics</subject><subject>Skin Neoplasms - metabolism</subject><subject>Skin Neoplasms - pathology</subject><subject>Tissue Array Analysis</subject><subject>Transcription factor</subject><subject>Transcription factors</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Up-Regulation</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqFUk1v1DAQtRCIbgv_AKFInBNm4o84FyRUFYpUgVSoxM1ynNnWS9Ze7OyK_nu8bGmBC6eR5-PN83vD2AuEBgHV61XjbJhoblpA0QBvAPERW6Du2rrrNTxmC-Agaq65PGLHOa8AQIpOPmVHrVCyU1Iu2OXl1cevbeVzFXeU6McmUc40Vj5Ua5psiGtbOZqmXNkwltTo7Uy5mm_Ip2rtr5OdfQy_ij7sbC6PZ-zJ0k6Znt_FE3b17uzL6Xl98en9h9O3F7WTQs21WkrtpEOttHYDDLzjeuQDdq0GO1oYNboBlYbeDr0FybXqxl70HJSgQQl-wt4ccDfboRBzFOZkJ7NJfm3TrYnWm78rwd-Y67gzAgSiwgLw6g4gxe9byrNZxW0KhbNBWSRSLXaqdIlDl0sx50TL-w0IZu-EWZmDE2bvhAFuihNl7OWf7O6Hfkv_QJ-KRjtPyWTnKbgicSI3mzH6_234F8BNPnhnp290S_nhLya3Bszn_TXsjwEFAEql-E_VDrDA</recordid><startdate>20140628</startdate><enddate>20140628</enddate><creator>Boregowda, Rajeev K</creator><creator>Olabisi, Oyenike O</creator><creator>Abushahba, Walid</creator><creator>Jeong, Byeong-Seon</creator><creator>Haenssen, Keneshia K</creator><creator>Chen, Wenjin</creator><creator>Chekmareva, Marina</creator><creator>Lasfar, Ahmed</creator><creator>Foran, David J</creator><creator>Goydos, James S</creator><creator>Cohen-Solal, Karine A</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>5PM</scope></search><sort><creationdate>20140628</creationdate><title>RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion</title><author>Boregowda, Rajeev K ; Olabisi, Oyenike O ; Abushahba, Walid ; Jeong, Byeong-Seon ; Haenssen, Keneshia K ; Chen, Wenjin ; Chekmareva, Marina ; Lasfar, Ahmed ; Foran, David J ; Goydos, James S ; Cohen-Solal, Karine A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c546t-6f58c5c18688cb0b3738d3b17280ada0d81cb16809ab9a053867d9493064eb643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bone cancer</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation</topic><topic>Cholecalciferol (Vitamin D3)</topic><topic>Cholecalciferol - pharmacology</topic><topic>Core Binding Factor Alpha 1 Subunit - genetics</topic><topic>Core Binding Factor Alpha 1 Subunit - metabolism</topic><topic>FAK</topic><topic>Focal Adhesion Kinase 1 - metabolism</topic><topic>Gene amplification</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Grants</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kinases</topic><topic>Matrix Metalloproteinase 13 - genetics</topic><topic>Medical research</topic><topic>Melanoma</topic><topic>Melanoma - genetics</topic><topic>Melanoma - metabolism</topic><topic>Melanoma - pathology</topic><topic>Metastasis</topic><topic>Migration</topic><topic>Motility</topic><topic>Neoplasm Invasiveness</topic><topic>Promoter Regions, Genetic</topic><topic>Proteins</topic><topic>RNA Interference</topic><topic>RUNX2</topic><topic>Signal Transduction</topic><topic>Skin Neoplasms - genetics</topic><topic>Skin Neoplasms - metabolism</topic><topic>Skin Neoplasms - pathology</topic><topic>Tissue Array Analysis</topic><topic>Transcription factor</topic><topic>Transcription factors</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boregowda, Rajeev K</creatorcontrib><creatorcontrib>Olabisi, Oyenike O</creatorcontrib><creatorcontrib>Abushahba, Walid</creatorcontrib><creatorcontrib>Jeong, Byeong-Seon</creatorcontrib><creatorcontrib>Haenssen, Keneshia K</creatorcontrib><creatorcontrib>Chen, Wenjin</creatorcontrib><creatorcontrib>Chekmareva, Marina</creatorcontrib><creatorcontrib>Lasfar, Ahmed</creatorcontrib><creatorcontrib>Foran, David J</creatorcontrib><creatorcontrib>Goydos, James S</creatorcontrib><creatorcontrib>Cohen-Solal, Karine A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boregowda, Rajeev K</au><au>Olabisi, Oyenike O</au><au>Abushahba, Walid</au><au>Jeong, Byeong-Seon</au><au>Haenssen, Keneshia K</au><au>Chen, Wenjin</au><au>Chekmareva, Marina</au><au>Lasfar, Ahmed</au><au>Foran, David J</au><au>Goydos, James S</au><au>Cohen-Solal, Karine A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2014-06-28</date><risdate>2014</risdate><volume>348</volume><issue>1</issue><spage>61</spage><epage>70</epage><pages>61-70</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Abstract In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>24657655</pmid><doi>10.1016/j.canlet.2014.03.011</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3835 |
| ispartof | Cancer letters, 2014-06, Vol.348 (1), p.61-70 |
| issn | 0304-3835 1872-7980 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4041161 |
| source | Elsevier |
| subjects | Bone cancer Cell growth Cell Line, Tumor Cell Movement - drug effects Cell Proliferation Cholecalciferol (Vitamin D3) Cholecalciferol - pharmacology Core Binding Factor Alpha 1 Subunit - genetics Core Binding Factor Alpha 1 Subunit - metabolism FAK Focal Adhesion Kinase 1 - metabolism Gene amplification Gene expression Gene Expression Regulation, Neoplastic Grants Hematology, Oncology and Palliative Medicine Humans Immunohistochemistry Kinases Matrix Metalloproteinase 13 - genetics Medical research Melanoma Melanoma - genetics Melanoma - metabolism Melanoma - pathology Metastasis Migration Motility Neoplasm Invasiveness Promoter Regions, Genetic Proteins RNA Interference RUNX2 Signal Transduction Skin Neoplasms - genetics Skin Neoplasms - metabolism Skin Neoplasms - pathology Tissue Array Analysis Transcription factor Transcription factors Transcription, Genetic Transfection Up-Regulation |
| title | RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion |
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