Vaccinia virus exhibits cell-type-dependent entry characteristics

Abstract Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a β-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was stro...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2009-03, Vol.385 (2), p.383-391
Main Authors: Whitbeck, J. Charles, Foo, Chwan-Hong, Ponce de Leon, Manuel, Eisenberg, Roselyn J, Cohen, Gary H
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - metabolism
Bafilomycin
beta-Galactosidase - metabolism
Cell Line
Cercopithecus aethiops
Cricetinae
HeLa Cells
Heparin
Heparin - metabolism
Humans
Hydrogen-Ion Concentration
Infectious Disease
Mice
Receptors, Virus - metabolism
Reporter virus
Vaccinia
Vaccinia virus
Vaccinia virus - physiology
Vero Cells
Virus Attachment
Virus entry
Virus Inactivation
Virus Internalization
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Summary:Abstract Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a β-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin whereas entry into Vero and BSC-1 cells was unaffected. Bafilomycin also exhibited variable and cell-type-specific effects on VV entry. Entry into B78H1 and BSC-1 cells was strongly inhibited by bafilomycin whereas entry into Vero and HeLa cells was only partially inhibited suggesting the co-existence of both pH-dependent and pH-independent VV entry pathways in these cell types. Finally, entry into HeLa, B78H1, L and BSC-1 cells exhibited a lag of 6–9 min whereas this delay was undetectable in Vero cells. Our results suggest that VV exploits multiple cell attachment and entry pathways allowing it to infect a broad range of cells.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2008.12.029