Improving oral human papillomavirus detection using toothbrush sampling in HIV-positive men who have sex with men

• Published in: Journal of clinical microbiology 2014-06, Vol.52 (6), p.2206-2209
• Main Authors: Ong, Jason J, Read, Tim, Chen, Marcus, Walker, Sandra, Law, Matthew, Bradshaw, Catriona, Garland, Suzanne M, Tabrizi, Sepehr N, Cornall, Alyssa, Grulich, Andrew, Hocking, Jane, Fairley, Christopher K
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Diagnosis, Oral - methods; HIV Infections - complications; Homosexuality, Male; Human immunodeficiency virus; Human papillomavirus; Humans; Male; Middle Aged; Papillomaviridae - isolation & purification; Papillomavirus Infections - diagnosis; Sensitivity and Specificity; Specimen Handling - methods; Virology; Young Adult
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.00286-14

Pre- and postabrasion oral rinse samples (ORS) and a toothbrush sample detected human papillomavirus (HPV) DNA in at least one sample among 45 (26%) of 173 HIV-positive men who have sex with men. There was moderate agreement for HPV genotype detection between the preabrasion and postabrasion ORS (κ = 0.49; 95% confidence interval [CI], 0.37 to 0.61). There was good agreement between postabrasion ORS and toothbrushes (κ = 0.70; 95% CI, 0.60 to 0.80). The sensitivities for HPV genotypes detected were 80% (95% CI, 69 to 88) for preabrasion ORS, 65% (95% CI, 54 to 76) for postabrasion ORS, and 75% (95% CI, 63 to 84) for toothbrushes.