Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matri...

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Bibliographic Details
Published in:Journal of clinical microbiology 2014-06, Vol.52 (6), p.2196-2198
Main Authors: Desnos-Ollivier, Marie, Blanc, Catherine, Garcia-Hermoso, Dea, Hoinard, Damien, Alanio, Alexandre, Dromer, Françoise
Format: Article
Language:English
Subjects:
Carbon - metabolism
Cluster Analysis
Diagnostic Tests, Routine - methods
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
Humans
Life Sciences
Microbiology and Parasitology
Molecular Sequence Data
Mycological Typing Techniques
Mycology
Mycoses - diagnosis
Mycoses - microbiology
Phylogeny
Saccharomycetales - chemistry
Saccharomycetales - classification
Saccharomycetales - genetics
Saccharomycetales - isolation & purification
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprints that enable the identification of S. clavata, M. capitatus, and Galactomyces candidus to the species level.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00039-14