Cytotoxicity assessment of ZnO nanoparticles on human epidermal cells

• Published in: Molecular cytogenetics 2014-01, Vol.7 (Suppl 1), p.P81-P81
• Main Authors: Patel, Pal, Kansara, Krupa, Shah, Darshini, Vallabani, NV, Shukla, Ritesh K, Singh, Sanjay, Dhawan, Alok, Kumar, Ashutosh
• Format: Article
• Language: English
• Subjects: Poster Presentation
• ISSN: 1755-8166; 1755-8166
• DOI: 10.1186/1755-8166-7-S1-P81

Background: Nanotechnology is growing rapidly worldwide and engineered nanoparticles have found tremendous applications in consumer and industrial products. Metal oxide nanoparticles (NPs), especially zinc oxide (ZnO), are widely used in cosmetics, catalysis, electronics, biosensors, medicine, paints, food packaging and imaging. As, the ZnO NPs have major application in cosmetics, their exposure will mainly be through the skin, which is the largest organ of the body and could serve as an important portal route for entry. Therefore, the present study was carried out to assess the cytotoxicity of ZnO NPs on human epidermal cells (A431). Materials and methods: The average hydrodynamic size, size distribution, zeta potential and stability of ZnO NPs were determined by dynamic light scattering (DLS). The detection for the internalization of ZnO NPs was carried out using flow cytometry. Cytotoxicity response was assessed by neutral red uptake (NRU) and MTT [3-(4, 5-dimethylthiazoyl-2-yl)-2, 5-diphenyltetrazolium bromide] assays. Further, to analyze the ability of ZnO NPs to induce the reactive oxygen species (ROS) generation and oxidative damage, 2, 7-dichlorofluorescein diacetate (DCFDA) dye was used. The effect of ZnO NPs in progression of cell cycle was also assessed using propidium iodide dye by flow cytometer. Results: The mean hydrodynamic diameter and zeta potential of ZnO NPs in DMEM with 10% FBS was 30.95 plus or minus 3.72 nm and -12.8 plus or minus 0.6 mV respectively. In flow cytometry, a concentration dependent significant (p