MicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori

The growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production. Here, aided by next-generation sequencing and microarry assay, we profile 1,229 microRNAs (miRNAs), in...

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Bibliographic Details
Published in:BMC genomics 2014-05, Vol.15 (1), p.410-410, Article 410
Main Authors: Li, Jisheng, Cai, Yimei, Ye, Lupeng, Wang, Shaohua, Che, Jiaqian, You, Zhengying, Yu, Jun, Zhong, Boxiong
Format: Article
Language:English
Subjects:
Animal sciences
Animals
Bombyx - genetics
Bombyx - growth & development
Bombyx - metabolism
Bombyx mori
Data processing
Gene expression
Genes
Genetic aspects
Genomes
Genomics
High-Throughput Nucleotide Sequencing
Larva - metabolism
Lasers
Mass spectrometry
MicroRNAs
MicroRNAs - metabolism
Oligonucleotide Array Sequence Analysis
Ontology
Protein synthesis
Proteins
Sequence Analysis, RNA
Signal transduction
Silk - metabolism
Technological change
Transcriptome
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Summary:The growth and development of the posterior silk gland and the biosynthesis of the silk core protein at the fifth larval instar stage of Bombyx mori are of paramount importance for silk production. Here, aided by next-generation sequencing and microarry assay, we profile 1,229 microRNAs (miRNAs), including 728 novel miRNAs and 110 miRNA/miRNA* duplexes, of the posterior silk gland at the fifth larval instar. Target gene prediction yields 14,222 unique target genes from 1,195 miRNAs. Functional categorization classifies the targets into complex pathways that include both cellular and metabolic processes, especially protein synthesis and processing. The enrichment of target genes in the ribosome-related pathway indicates that miRNAs may directly regulate translation. Our findings pave a way for further functional elucidation of these miRNAs and their targets in silk production.
ISSN:1471-2164
1471-2164
DOI:10.1186/1471-2164-15-410