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Development and validation of a bioreactor system for dynamic loading and mechanical characterization of whole human intervertebral discs in organ culture

Abstract Intervertebral disc (IVD) degeneration is a common cause of back pain, and attempts to develop therapies are frustrated by lack of model systems that mimic the human condition. Human IVD organ culture models can address this gap, yet current models are limited since vertebral endplates are...

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Published in:Journal of biomechanics 2014-06, Vol.47 (9), p.2095-2101
Main Authors: Walter, B.A, Illien-Jünger, S, Nasser, P.R, Hecht, A.C, Iatridis, J.C
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description Abstract Intervertebral disc (IVD) degeneration is a common cause of back pain, and attempts to develop therapies are frustrated by lack of model systems that mimic the human condition. Human IVD organ culture models can address this gap, yet current models are limited since vertebral endplates are removed to maintain cell viability, physiological loading is not applied, and mechanical behaviors are not measured. This study aimed to (i) establish a method for isolating human IVDs from autopsy with intact vertebral endplates, and (ii) develop and validate an organ culture loading system for human or bovine IVDs. Human IVDs with intact endplates were isolated from cadavers within 48 h of death and cultured for up to 21 days. IVDs remained viable with ~80% cell viability in nucleus and annulus regions. A dynamic loading system was designed and built with the capacity to culture 9 bovine or 6 human IVDs simultaneously while applying simulated physiologic loads (maximum force: 4 kN) and measuring IVD mechanical behaviors. The loading system accurately applied dynamic loading regimes (RMS error
doi_str_mv 10.1016/j.jbiomech.2014.03.015
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subjects Animals
Back pain
Biodegradable materials
Biomechanical Phenomena
Bioreactor loading system
Bioreactors
Bovine
Cattle
Child
Culture
Design
Dynamical systems
Dynamics
Female
Human
Humans
Intervertebral disc
Intervertebral Disc - physiology
Intervertebral discs
Loads (forces)
Male
Mechanical properties
Middle Aged
Muscular system
Organ culture
Organ Culture Techniques
Organs
Physical Medicine and Rehabilitation
Reproducibility of Results
Studies
Tissue engineering
title Development and validation of a bioreactor system for dynamic loading and mechanical characterization of whole human intervertebral discs in organ culture
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