Effects of bile acids on cyclooxygenase-2 expression in a rat model of duodenoesophageal anastomosis

AIM:To examine the expression of cyclooxygenase-2(COX-2)and prostaglandin E2(PGE2)in rat esophageal lesions induced by reflux of duodenal contents.METHODS:Thirty 8-week-old male Wistar rats were exposed to duodenal content esophageal reflux.All animals underwent an esophagoduodenal anastomosis(EDA)w...

Full description

Saved in:
Bibliographic Details
Published in:World journal of gastroenterology : WJG 2014-06, Vol.20 (21), p.6541-6546
Main Author: Hashimoto, Naoki
Format: Article
Language:English
Subjects:
acids
Anastomosis, Surgical
Animals
Bile
Bile Acids and Salts - chemistry
Cyclooxygenase 2 - metabolism
Cyclooxygenase-2
Dinoprostone - metabolism
Disease Models, Animal
Duodenum - pathology
Esoph
Esophageal Neoplasms - metabolism
Esophagus - pathology
Gene Expression Regulation, Enzymologic
Immunohistochemistry
Male
Neovascularization, Pathologic
Proliferating Cell Nuclear Antigen - metabolism
Prostaglandin
Rats
Rats, Wistar
Research Report
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:AIM:To examine the expression of cyclooxygenase-2(COX-2)and prostaglandin E2(PGE2)in rat esophageal lesions induced by reflux of duodenal contents.METHODS:Thirty 8-week-old male Wistar rats were exposed to duodenal content esophageal reflux.All animals underwent an esophagoduodenal anastomosis(EDA)with total gastrectomy to elicit chronic esophagitis.In ten rats sham operations with only a midline laparotomy were performed(Control).The rats were sacrificed at the 40th week,their esophagi were taken for hematoxylin and eosin staining and for examination of expression of COX2,PGE2,and proliferating cell nuclear antigen(PCNA),and total bile acids in the esophageal lumen was measured.RESULTS:After 40 wk of reflux,columnar dysplasia,squamous cell carcinoma and adenocarcinoma were observed.Total bile acids in the esophageal lumen were significantly increased in the EDA group compared with the sham operated rats.PCNA labelling index and esophageal tissue PGE2 levels were higher in dysplastic and cancer tissues than in control tissues.Overexpression of COX2 was observed in dysplastic and cancer tissues.CONCLUSION:Reflux of duodenal contents induces COX2 expression and increases prostaglandin synthesis in dysplastic and cancer tissues.This result suggests a possible mechanism by which bile acids promote esophageal cancer.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v20.i21.6541