Circadian Modulation of the Cl− Equilibrium Potential in the Rat Suprachiasmatic Nuclei

• Published in: BioMed research international 2014-01, Vol.2014 (2014), p.1-15
• Main Authors: Alamilla, Javier, Perez-Burgos, Azucena, Quinto, Daniel, Aguilar-Roblero, Raúl
• Format: Article
• Language: English
• Subjects: Acids; Animals; Brain; Circadian Clocks - genetics; Circadian Clocks - physiology; Circadian rhythm; Equilibrium; Experiments; gamma-Aminobutyric Acid - metabolism; Neurons; Neurons - metabolism; Neurosciences; Patch-Clamp Techniques; Rats; Receptors, GABA-A - metabolism; Rodents; Solute Carrier Family 12, Member 2 - metabolism; Suprachiasmatic Nucleus - metabolism; Synaptic Transmission - genetics
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/424982

The suprachiasmatic nuclei (SCN) constitute a circadian clock in mammals, where γ-amino-butyric acid (GABA) neurotransmission prevails and participates in different aspects of circadian regulation. Evidence suggests that GABA has an excitatory function in the SCN in addition to its typical inhibitory role. To examine this possibility further, we determined the equilibrium potential of GABAergic postsynaptic currents (EGABA) at different times of the day and in different regions of the SCN, using either perforated or whole cell patch clamp. Our results indicate that during the day most neurons in the dorsal SCN have an EGABA close to −30 mV while in the ventral SCN they have an EGABA close to −60 mV; this difference reverses during the night, in the dorsal SCN neurons have an EGABA of −60 mV and in the ventral SCN they have an EGABA of −30 mV. The depolarized equilibrium potential can be attributed to the activity of the Na(+)-K(+)-2Cl(−) (NKCC) cotransporter since the equilibrium potential becomes more negative following addition of the NKCC blocker bumetanide. Our results suggest an excitatory role for GABA in the SCN and further indicate both time (day versus night) and regional (dorsal versus ventral) modulation of EGABA in the SCN.