Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection

This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence....

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-11
Main Authors: Olivera, Noelia, Castuma, Celina E., Hozbor, Daniela, Gaillard, María E., Rumbo, Martín, Gómez, Ricardo M.
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - immunology
Bordetella pertussis
Bordetella pertussis - immunology
Bronchoalveolar Lavage Fluid
Cholera toxin
Cholera Toxin - immunology
Cloning
Cytokines - genetics
Cytokines - metabolism
E coli
Female
Fimbria
Fimbriae Proteins - immunology
Glycerol
Health aspects
Immunity, Humoral
Immunization
Immunoglobulin A - blood
Immunoglobulin G - blood
Infections
Lung - metabolism
Lung - microbiology
Lung - pathology
Mice, Inbred BALB C
Plasmids
Prevention
Proteins
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - isolation & purification
RNA, Messenger - genetics
RNA, Messenger - metabolism
Vaccines
Virulence Factors, Bordetella - immunology
Whooping cough
Whooping Cough - blood
Whooping Cough - immunology
Whooping Cough - microbiology
Whooping Cough - prevention & control
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Summary:This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence. The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/421486