International study on inter-reader variability for circulating tumor cells in breast cancer

Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement. CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-...

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Published in:Breast cancer research : BCR 2014-04, Vol.16 (2), p.R43-R43, Article R43
Main Authors: Ignatiadis, Michail, Riethdorf, Sabine, Bidard, François-Clement, Vaucher, Isabelle, Khazour, Mustapha, Rothé, Françoise, Metallo, Jessica, Rouas, Ghizlane, Payne, Rachel E, Coombes, Raoul, Teufel, Ingrid, Andergassen, Ulrich, Apostolaki, Stella, Politaki, Eleni, Mavroudis, Dimitris, Bessi, Silvia, Pestrin, Marta, Di Leo, Angelo, Campion, Michael, Reinholz, Monica, Perez, Edith, Piccart, Martine, Borgen, Elin, Naume, Bjorn, Jimenez, Jose, Aura, Claudia, Zorzino, Laura, Cassatella, Maria, Sandri, Maria, Mostert, Bianca, Sleijfer, Stefan, Kraan, Jaco, Janni, Wolfgang, Fehm, Tanja, Rack, Brigitte, Terstappen, Leon, Repollet, Madeline, Pierga, Jean-Yves, Miller, Craig, Sotiriou, Christos, Michiels, Stefan, Pantel, Klaus
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Language:English
Subjects:
Breast Neoplasms - blood
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Count - instrumentation
Cell Count - standards
Female
Humans
International Cooperation
Laboratories - standards
Medical Oncology - instrumentation
Medical Oncology - standards
Neoplasm Metastasis
Neoplastic Cells, Circulating - metabolism
Neoplastic Cells, Circulating - pathology
Receptor, ErbB-2 - metabolism
Reference Standards
Reproducibility of Results
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cited_by cdi_FETCH-LOGICAL-c469t-8dbf2a3236db94682a0970e6f9867c354bb06ed5229e09297473746413e8e36d3
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container_end_page R43
container_issue 2
container_start_page R43
container_title Breast cancer research : BCR
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creator Ignatiadis, Michail
Riethdorf, Sabine
Bidard, François-Clement
Vaucher, Isabelle
Khazour, Mustapha
Rothé, Françoise
Metallo, Jessica
Rouas, Ghizlane
Payne, Rachel E
Coombes, Raoul
Teufel, Ingrid
Andergassen, Ulrich
Apostolaki, Stella
Politaki, Eleni
Mavroudis, Dimitris
Bessi, Silvia
Pestrin, Marta
Di Leo, Angelo
Campion, Michael
Reinholz, Monica
Perez, Edith
Piccart, Martine
Borgen, Elin
Naume, Bjorn
Jimenez, Jose
Aura, Claudia
Zorzino, Laura
Cassatella, Maria
Sandri, Maria
Mostert, Bianca
Sleijfer, Stefan
Kraan, Jaco
Janni, Wolfgang
Fehm, Tanja
Rack, Brigitte
Terstappen, Leon
Repollet, Madeline
Pierga, Jean-Yves
Miller, Craig
Sotiriou, Christos
Michiels, Stefan
Pantel, Klaus
description Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement. CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test. For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P 
doi_str_mv 10.1186/bcr3647
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Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement. CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test. For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P &lt; 0.001) and from M0 and &lt;3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P &lt; 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90). The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. 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Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P &lt; 0.001) and from M0 and &lt;3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P &lt; 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90). The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. 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blood</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Cell Count - instrumentation</topic><topic>Cell Count - standards</topic><topic>Female</topic><topic>Humans</topic><topic>International Cooperation</topic><topic>Laboratories - standards</topic><topic>Medical Oncology - instrumentation</topic><topic>Medical Oncology - standards</topic><topic>Neoplasm Metastasis</topic><topic>Neoplastic Cells, Circulating - metabolism</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Receptor, ErbB-2 - metabolism</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ignatiadis, Michail</creatorcontrib><creatorcontrib>Riethdorf, Sabine</creatorcontrib><creatorcontrib>Bidard, François-Clement</creatorcontrib><creatorcontrib>Vaucher, Isabelle</creatorcontrib><creatorcontrib>Khazour, Mustapha</creatorcontrib><creatorcontrib>Rothé, Françoise</creatorcontrib><creatorcontrib>Metallo, Jessica</creatorcontrib><creatorcontrib>Rouas, Ghizlane</creatorcontrib><creatorcontrib>Payne, Rachel E</creatorcontrib><creatorcontrib>Coombes, Raoul</creatorcontrib><creatorcontrib>Teufel, Ingrid</creatorcontrib><creatorcontrib>Andergassen, Ulrich</creatorcontrib><creatorcontrib>Apostolaki, Stella</creatorcontrib><creatorcontrib>Politaki, Eleni</creatorcontrib><creatorcontrib>Mavroudis, Dimitris</creatorcontrib><creatorcontrib>Bessi, Silvia</creatorcontrib><creatorcontrib>Pestrin, Marta</creatorcontrib><creatorcontrib>Di Leo, Angelo</creatorcontrib><creatorcontrib>Campion, Michael</creatorcontrib><creatorcontrib>Reinholz, Monica</creatorcontrib><creatorcontrib>Perez, Edith</creatorcontrib><creatorcontrib>Piccart, Martine</creatorcontrib><creatorcontrib>Borgen, Elin</creatorcontrib><creatorcontrib>Naume, Bjorn</creatorcontrib><creatorcontrib>Jimenez, Jose</creatorcontrib><creatorcontrib>Aura, Claudia</creatorcontrib><creatorcontrib>Zorzino, Laura</creatorcontrib><creatorcontrib>Cassatella, Maria</creatorcontrib><creatorcontrib>Sandri, Maria</creatorcontrib><creatorcontrib>Mostert, Bianca</creatorcontrib><creatorcontrib>Sleijfer, Stefan</creatorcontrib><creatorcontrib>Kraan, Jaco</creatorcontrib><creatorcontrib>Janni, Wolfgang</creatorcontrib><creatorcontrib>Fehm, Tanja</creatorcontrib><creatorcontrib>Rack, Brigitte</creatorcontrib><creatorcontrib>Terstappen, Leon</creatorcontrib><creatorcontrib>Repollet, Madeline</creatorcontrib><creatorcontrib>Pierga, Jean-Yves</creatorcontrib><creatorcontrib>Miller, Craig</creatorcontrib><creatorcontrib>Sotiriou, Christos</creatorcontrib><creatorcontrib>Michiels, Stefan</creatorcontrib><creatorcontrib>Pantel, Klaus</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Breast cancer research : BCR</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ignatiadis, Michail</au><au>Riethdorf, Sabine</au><au>Bidard, François-Clement</au><au>Vaucher, Isabelle</au><au>Khazour, Mustapha</au><au>Rothé, Françoise</au><au>Metallo, Jessica</au><au>Rouas, Ghizlane</au><au>Payne, Rachel E</au><au>Coombes, Raoul</au><au>Teufel, Ingrid</au><au>Andergassen, Ulrich</au><au>Apostolaki, Stella</au><au>Politaki, Eleni</au><au>Mavroudis, Dimitris</au><au>Bessi, Silvia</au><au>Pestrin, Marta</au><au>Di Leo, Angelo</au><au>Campion, Michael</au><au>Reinholz, Monica</au><au>Perez, Edith</au><au>Piccart, Martine</au><au>Borgen, Elin</au><au>Naume, Bjorn</au><au>Jimenez, Jose</au><au>Aura, Claudia</au><au>Zorzino, Laura</au><au>Cassatella, Maria</au><au>Sandri, Maria</au><au>Mostert, Bianca</au><au>Sleijfer, Stefan</au><au>Kraan, Jaco</au><au>Janni, Wolfgang</au><au>Fehm, Tanja</au><au>Rack, Brigitte</au><au>Terstappen, Leon</au><au>Repollet, Madeline</au><au>Pierga, Jean-Yves</au><au>Miller, Craig</au><au>Sotiriou, Christos</au><au>Michiels, Stefan</au><au>Pantel, Klaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>International study on inter-reader variability for circulating tumor cells in breast cancer</atitle><jtitle>Breast cancer research : BCR</jtitle><addtitle>Breast Cancer Res</addtitle><date>2014-04-23</date><risdate>2014</risdate><volume>16</volume><issue>2</issue><spage>R43</spage><epage>R43</epage><pages>R43-R43</pages><artnum>R43</artnum><issn>1465-542X</issn><issn>1465-5411</issn><eissn>1465-542X</eissn><abstract>Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement. CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test. For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P &lt; 0.001) and from M0 and &lt;3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P &lt; 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90). The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. Continuous training and independent image review are required.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>24758318</pmid><doi>10.1186/bcr3647</doi><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1465-542X
ispartof Breast cancer research : BCR, 2014-04, Vol.16 (2), p.R43-R43, Article R43
issn 1465-542X
1465-5411
1465-542X
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4052944
source PubMed Central
subjects Breast Neoplasms - blood
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Count - instrumentation
Cell Count - standards
Female
Humans
International Cooperation
Laboratories - standards
Medical Oncology - instrumentation
Medical Oncology - standards
Neoplasm Metastasis
Neoplastic Cells, Circulating - metabolism
Neoplastic Cells, Circulating - pathology
Receptor, ErbB-2 - metabolism
Reference Standards
Reproducibility of Results
title International study on inter-reader variability for circulating tumor cells in breast cancer
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