Anticoagulant Activity of Polyphenolic-Polysaccharides Isolated from Melastoma malabathricum L.

Melastoma malabathricum Linn. is a perennial traditional medicine plants that grows abundantly throughout Asian countries. In this study, M. malabathricum Linn. leaf hot water crude extract with anticoagulant activity was purified through solid phase extraction cartridge and examined for the bioacti...

Full description

Saved in:
Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2014-01, Vol.2014 (2014), p.1-8
Main Authors: Mohd Tohit, Eusni Rahayu, Abdullah, Janna Ong, Abas, Faridah, Khoo, Li Teng, Hamid, Muhajir
Format: Article
Language:English
Subjects:
Anticoagulants
Anticoagulants (Medicine)
Automation
Blood coagulation
Carbohydrates
Clotting
Constituents
Flavonoids
Health aspects
Herbal medicine
Hexose
Leaves
Lythrum salicaria
Medicinal plants
Medicine, Botanic
Medicine, Herbal
Melastoma malabathricum
Phenols
Polymers
Polysaccharides
Properties
Rhamnogalacturonan
Rhamnose
Saccharides
Sodium
Sugar
Thromboplastin
Thrombosis
Water purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Melastoma malabathricum Linn. is a perennial traditional medicine plants that grows abundantly throughout Asian countries. In this study, M. malabathricum Linn. leaf hot water crude extract with anticoagulant activity was purified through solid phase extraction cartridge and examined for the bioactive chemical constituents on blood coagulation reaction. The SPE purified fractions were, respectively, designated as F1, F2, F3, and F4, and each was subjected to the activated partial thromboplastin time (APTT) anticoagulant assay. Active anticoagulant fractions (F1, F2, and F3) were subjected to chemical characterisation evaluation. Besides, neutral sugar for carbohydrate part was also examined. F1, F2, and F3 were found to significantly prolong the anticoagulant activities in the following order, F1 > F2 > F3 , in a dose dependent manner. In addition, carbohydrate, hexuronic acid, and polyphenolic moiety were measured for the active anticoagulant fractions (F1, F2, and F3). The characterisation of chemical constituents revealed that all these three fractions contained acidic polysaccharides (rhamnogalacturonan, homogalacturonan, and rhamnose hexose-pectic type polysaccharide) and polyphenolics. Hence, it was concluded that the presence of high hexuronic acids and polysaccharides, as well as polyphenolics in traditional medicinal plant, M. malabathricum, played a role in prolonging blood clotting in the intrinsic pathway.
ISSN:1741-427X
1741-4288
DOI:10.1155/2014/614273