Molecular strategies for detecting chromosomal translocations in soft tissue tumors (Review)

Approximately one third of soft tissue tumors are characterized by chromosomal aberrations, in particular, translocations and amplifications, which appear to be highly specific. The identification of fusion transcripts not only supports the diagnosis, but provides the basis for the development of no...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular medicine 2014-06, Vol.33 (6), p.1379-1391
Main Authors: CERRONE, MARGHERITA, CANTILE, MONICA, COLLINA, FRANCESCA, MARRA, LAURA, LIGUORI, GIUSEPPINA, FRANCO, RENATO, DE CHIARA, ANNAROSARIA, BOTTI, GERARDO
Format: Article
Language:English
Subjects:
Analysis
Biopsy
chromosomal translocations
Chromosome abnormalities
Chromosomes
Cloning
Diagnosis
Fibroblasts
Genes
Hybridization
Laboratories
Methods
molecular analyses
Nanotechnology
Sarcoma
soft tissue tumors
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Approximately one third of soft tissue tumors are characterized by chromosomal aberrations, in particular, translocations and amplifications, which appear to be highly specific. The identification of fusion transcripts not only supports the diagnosis, but provides the basis for the development of novel therapeutic strategies aimed at blocking the aberrant activity of chimeric proteins. Molecular biology, and in particular, cytogenetic and qualitative and quantitative polymerase chain reaction technologies, allow with high efficiency and specificity, the determination of specific fusion transcripts resulting from chromosomal translocations, as well as the analysis of gene amplifications. In this review, various molecular techniques that allow the identification of translocations and consequent fusion transcripts generated are discussed in the broad spectrum of soft tissue tumors.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2014.1726