Effect of long-term dietary arginyl-fructose (AF) on hyperglycemia and HbA1c in diabetic db/db mice

• Published in: International journal of molecular sciences 2014-05, Vol.15 (5), p.8352-8359
• Main Authors: Lee, Kwang-Hyoung, Ha, Kyoung-Soo, Jo, Sung-Hoon, Lee, Chong M, Kim, Young-Cheul, Chung, Kwang-Hoe, Kwon, Young-In
• Format: Article
• Language: English
• Subjects: Animals; Blood Glucose - drug effects; Diabetes; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - diet therapy; Dietary Supplements - analysis; Enzymes; Fructose - analogs & derivatives; Fructose - therapeutic use; Glucose; Glycated Hemoglobin - analysis; Glycoside Hydrolase Inhibitors - chemistry; Glycoside Hydrolase Inhibitors - therapeutic use; Hyperglycemia; Hyperglycemia - blood; Hyperglycemia - complications; Hyperglycemia - diet therapy; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - therapeutic use; Male; Mice; Mice, Inbred C57BL; Rodents
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms15058352

We have previously reported that Amadori compounds exert anti-diabetic effects by lowering sucrose-induced hyperglycemia in normal Sprague-Dawley rats. In the present study we extended our recent findings to evaluate whether α-glucosidase inhibitor arginyl-fructose (AF) lowers blood glucose level in diabetic db/db mice, a genetic model for type 2 diabetes. The db/db mice were randomly assigned to high-carbohydrate diets (66.1% corn starch) with and without AF (4% in the diet) for 6 weeks. Changes in body weight, blood glucose level, and food intake were measured daily for 42 days. Dietary supplementation of AF resulted in a significant decrease of blood glucose level (p < 0.001) and body weight (p < 0.001). The level of HbA1c, a better indicator of plasma glucose concentration over prolonged periods of time, was also significantly decreased for 6-week period (p < 0.001). Dietary treatment of acarbose® (0.04% in diet), a positive control, also significantly alleviated the level of blood glucose, HbA1c, and body weight. These results indicate that AF Maillard reaction product improves postprandial hyperglycemia by suppressing glucose absorption as well as decreasing HbA1c level.