Application of PCR-ELISA in Molecular Diagnosis

Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared t...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-6
Main Authors: Sue, Mei Jean, Yeap, Swee Keong, Omar, Abdul Rahman, Tan, Sheau Wei
Format: Article
Language:English
Subjects:
Animals
Biomedical research
Deoxyribonucleic acid
DNA
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Food contamination & poisoning
Genes
Humans
Hybridization
Innovations
Laboratories
Laboratory equipment
Medical research
Medicine, Experimental
Methods
Molecular diagnostic techniques
Molecular Diagnostic Techniques - methods
Polymerase chain reaction
Polymerase Chain Reaction - methods
Review
Studies
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Description
Summary:Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/653014