Application of PCR-ELISA in Molecular Diagnosis

Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared t...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-6
Main Authors: Sue, Mei Jean, Yeap, Swee Keong, Omar, Abdul Rahman, Tan, Sheau Wei
Format: Article
Language:English
Subjects:
Animals
Biomedical research
Deoxyribonucleic acid
DNA
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Food contamination & poisoning
Genes
Humans
Hybridization
Innovations
Laboratories
Laboratory equipment
Medical research
Medicine, Experimental
Methods
Molecular diagnostic techniques
Molecular Diagnostic Techniques - methods
Polymerase chain reaction
Polymerase Chain Reaction - methods
Review
Studies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c527t-cfc327aa71fae5b393b282e636c013f34b90c823c976705a178251cb9806e3333
cites cdi_FETCH-LOGICAL-c527t-cfc327aa71fae5b393b282e636c013f34b90c823c976705a178251cb9806e3333
container_end_page 6
container_issue 2014
container_start_page 1
container_title BioMed research international
container_volume 2014
creator Sue, Mei Jean
Yeap, Swee Keong
Omar, Abdul Rahman
Tan, Sheau Wei
description Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.
doi_str_mv 10.1155/2014/653014
format article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4058250</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A427023952</galeid><sourcerecordid>A427023952</sourcerecordid><originalsourceid>FETCH-LOGICAL-c527t-cfc327aa71fae5b393b282e636c013f34b90c823c976705a178251cb9806e3333</originalsourceid><addsrcrecordid>eNqNkd9rFDEQx4MotrR98llZ8EUs28tk8mPzIhxnawsnij-eQzbNXlP2kuvmVvG_N8fWo_pS52UG8uHDZL6EvAB6BiDEjFHgMymwtCfkkCHwWgKHp_sZ8YCc5HxLSzUgqZbPyQHjWgFyPCSz-WbTB2e3IcUqddXnxZf6fHn1dV6FWH1MvXdjb4fqfbCrmHLIx-RZZ_vsT-77Efl-cf5tcVkvP324WsyXtRNMbWvXOWTKWgWd9aJFjS1rmJcoHQXskLeauoah00oqKiyohglwrW6o9FjqiLybvJuxXftr5-N2sL3ZDGFth18m2WD-fonhxqzSD8OpKCpaBG_uBUO6G33emnXIzve9jT6N2YCQshES8X9QAcWpqSzo63_Q2zQOsVyiUFxpkIo_oFa29ybELpUV3U5q5pwpylALVqjTiXJDynnw3f53QM0uXLML10zhFvrVw4Ps2T9RFuDtBNyEeG1_hkdsLyfYF8R3dg_zpilC_A1e8q_1</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1547916746</pqid></control><display><type>article</type><title>Application of PCR-ELISA in Molecular Diagnosis</title><source>Open Access: Wiley-Blackwell Open Access Journals</source><source>Publicly Available Content Database</source><creator>Sue, Mei Jean ; Yeap, Swee Keong ; Omar, Abdul Rahman ; Tan, Sheau Wei</creator><contributor>Siafakas, Nikolaos</contributor><creatorcontrib>Sue, Mei Jean ; Yeap, Swee Keong ; Omar, Abdul Rahman ; Tan, Sheau Wei ; Siafakas, Nikolaos</creatorcontrib><description>Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2014/653014</identifier><identifier>PMID: 24971343</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Animals ; Biomedical research ; Deoxyribonucleic acid ; DNA ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes ; Food contamination &amp; poisoning ; Genes ; Humans ; Hybridization ; Innovations ; Laboratories ; Laboratory equipment ; Medical research ; Medicine, Experimental ; Methods ; Molecular diagnostic techniques ; Molecular Diagnostic Techniques - methods ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Review ; Studies</subject><ispartof>BioMed research international, 2014-01, Vol.2014 (2014), p.1-6</ispartof><rights>Copyright © 2014 Mei Jean Sue et al.</rights><rights>COPYRIGHT 2014 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2014 Mei Jean Sue et al. Mei Jean Sue et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2014 Mei Jean Sue et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-cfc327aa71fae5b393b282e636c013f34b90c823c976705a178251cb9806e3333</citedby><cites>FETCH-LOGICAL-c527t-cfc327aa71fae5b393b282e636c013f34b90c823c976705a178251cb9806e3333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1547916746/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1547916746?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24971343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Siafakas, Nikolaos</contributor><creatorcontrib>Sue, Mei Jean</creatorcontrib><creatorcontrib>Yeap, Swee Keong</creatorcontrib><creatorcontrib>Omar, Abdul Rahman</creatorcontrib><creatorcontrib>Tan, Sheau Wei</creatorcontrib><title>Application of PCR-ELISA in Molecular Diagnosis</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.</description><subject>Animals</subject><subject>Biomedical research</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes</subject><subject>Food contamination &amp; poisoning</subject><subject>Genes</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Innovations</subject><subject>Laboratories</subject><subject>Laboratory equipment</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Methods</subject><subject>Molecular diagnostic techniques</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Review</subject><subject>Studies</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNkd9rFDEQx4MotrR98llZ8EUs28tk8mPzIhxnawsnij-eQzbNXlP2kuvmVvG_N8fWo_pS52UG8uHDZL6EvAB6BiDEjFHgMymwtCfkkCHwWgKHp_sZ8YCc5HxLSzUgqZbPyQHjWgFyPCSz-WbTB2e3IcUqddXnxZf6fHn1dV6FWH1MvXdjb4fqfbCrmHLIx-RZZ_vsT-77Efl-cf5tcVkvP324WsyXtRNMbWvXOWTKWgWd9aJFjS1rmJcoHQXskLeauoah00oqKiyohglwrW6o9FjqiLybvJuxXftr5-N2sL3ZDGFth18m2WD-fonhxqzSD8OpKCpaBG_uBUO6G33emnXIzve9jT6N2YCQshES8X9QAcWpqSzo63_Q2zQOsVyiUFxpkIo_oFa29ybELpUV3U5q5pwpylALVqjTiXJDynnw3f53QM0uXLML10zhFvrVw4Ps2T9RFuDtBNyEeG1_hkdsLyfYF8R3dg_zpilC_A1e8q_1</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Sue, Mei Jean</creator><creator>Yeap, Swee Keong</creator><creator>Omar, Abdul Rahman</creator><creator>Tan, Sheau Wei</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>John Wiley &amp; Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Application of PCR-ELISA in Molecular Diagnosis</title><author>Sue, Mei Jean ; Yeap, Swee Keong ; Omar, Abdul Rahman ; Tan, Sheau Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-cfc327aa71fae5b393b282e636c013f34b90c823c976705a178251cb9806e3333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biomedical research</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes</topic><topic>Food contamination &amp; poisoning</topic><topic>Genes</topic><topic>Humans</topic><topic>Hybridization</topic><topic>Innovations</topic><topic>Laboratories</topic><topic>Laboratory equipment</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Methods</topic><topic>Molecular diagnostic techniques</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Review</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sue, Mei Jean</creatorcontrib><creatorcontrib>Yeap, Swee Keong</creatorcontrib><creatorcontrib>Omar, Abdul Rahman</creatorcontrib><creatorcontrib>Tan, Sheau Wei</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East &amp; Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sue, Mei Jean</au><au>Yeap, Swee Keong</au><au>Omar, Abdul Rahman</au><au>Tan, Sheau Wei</au><au>Siafakas, Nikolaos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of PCR-ELISA in Molecular Diagnosis</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>2014</volume><issue>2014</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>24971343</pmid><doi>10.1155/2014/653014</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2314-6133
ispartof BioMed research international, 2014-01, Vol.2014 (2014), p.1-6
issn 2314-6133
2314-6141
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4058250
source Open Access: Wiley-Blackwell Open Access Journals; Publicly Available Content Database
subjects Animals
Biomedical research
Deoxyribonucleic acid
DNA
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Food contamination & poisoning
Genes
Humans
Hybridization
Innovations
Laboratories
Laboratory equipment
Medical research
Medicine, Experimental
Methods
Molecular diagnostic techniques
Molecular Diagnostic Techniques - methods
Polymerase chain reaction
Polymerase Chain Reaction - methods
Review
Studies
title Application of PCR-ELISA in Molecular Diagnosis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T21%3A27%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Application%20of%20PCR-ELISA%20in%20Molecular%20Diagnosis&rft.jtitle=BioMed%20research%20international&rft.au=Sue,%20Mei%20Jean&rft.date=2014-01-01&rft.volume=2014&rft.issue=2014&rft.spage=1&rft.epage=6&rft.pages=1-6&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2014/653014&rft_dat=%3Cgale_pubme%3EA427023952%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c527t-cfc327aa71fae5b393b282e636c013f34b90c823c976705a178251cb9806e3333%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1547916746&rft_id=info:pmid/24971343&rft_galeid=A427023952&rfr_iscdi=true