Effect of Low-Level Laser on Bone Defects Treated with Bovine or Autogenous Bone Grafts : In Vivo Study in Rat Calvaria

• Published in: BioMed research international 2014-01, Vol.2014 (2014), p.1-9
• Main Authors: Sbrana, Michyele C., Cunha, Mércia Jussara da Silva, de Almeida, Ana Lúcia Pompéia Fraga, do Valle, Accácio L., de Oliveira, Paula G. F. P., Esper, Luis Augusto
• Format: Article
• Language: English
• Subjects: Animals; Blood clots; Bone diseases; Bone Transplantation; Bone-grafting; Care and treatment; Cattle; Defects; Dentistry; Knee; Lasers; Lasers in medicine; Low-Level Light Therapy; Male; Osteogenesis; Patient outcomes; Skull - pathology; Statistics as Topic; Studies; Transplantation, Autologous
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/104230

Objective. The purpose of this study was to histologically evaluate the effect of low-level laser (LLL) on the healing of critical size defects (CSD) in rat calvaria, filled with autogenous or inorganic bovine bone grafts. Methods. Sixty rats were divided into 6 groups (n=10): C (control—filled with blood clot), LLL (low-level laser—GaAlAs, λ 780 nm, 100 mW, 210 J/cm2, Φ 0.05 cm2; 6 J/point), AB (autogenous bone), ABL (autogenous bone + low-level laser), OB (inorganic bovine bone), and OBL (inorganic bovine bone + LLL). Material and Methods. The animals were killed after 30 days. Histological and histometric analyses were performed by light microscopy. Results. The groups irradiated with laser, LLL (47.67% ± 8.66%), ABL (39.15% ± 16.72%), and OBL (48.57% ± 28.22%), presented greater area of new bone formation than groups C (9.96% ± 4.50%), AB (30.98% ± 16.59%), and OB (11.36% ± 7.89%), which were not irradiated. Moreover, they were significantly better than group C (Kruskal-Wallis test followed by Dunn test, P