Survivals of mouse oocytes approach 100% after vitrification in 3-fold diluted media and ultra-rapid warming by an IR laser pulse

Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protectiv...

Full description

Saved in:
Bibliographic Details
Published in:Cryobiology 2014-06, Vol.68 (3), p.419-430
Main Authors: Jin, Bo, Kleinhans, F.W., Mazur, Peter
Format: Article
Language:English
Subjects:
Animals
Cell Survival
Cryo Jig
Cryopreservation - instrumentation
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents - metabolism
Dehydration
Equipment Design
Female
Functional survival
Ice - analysis
Infrared Rays
Laser
Lasers
Mice - embryology
Mice - physiology
Mouse oocytes and embryos
Oocytes - cytology
Oocytes - metabolism
Osmosis
Recrystallization of ice
Ultra-rapid warming
Vitrification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that they be cooled at very high rates to below −100°C. We report here that neither of these beliefs holds for mouse oocytes. Rather, we find that if mouse oocytes are suspended in media that produce considerable osmotic dehydration before vitrification and are subsequently warmed at ultra high rates (10,000,000°C/min) achieved by a laser pulse, nearly 100% will survive even when cooled rather slowly and when the concentration of solutes in the medium is only 1/3rd of standard.
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2014.03.005