Evidence of natural Wolbachia infections in field populations of Anopheles gambiae

• Published in: Nature communications 2014-06, Vol.5 (1), p.3985, Article 3985
• Main Authors: Baldini, Francesco, Segata, Nicola, Pompon, Julien, Marcenac, Perrine, Robert Shaw, W., Dabiré, Roch K., Diabaté, Abdoulaye, Levashina, Elena A., Catteruccia, Flaminia
• Format: Article
• Language: English
• Subjects: 45; 631/158/855; 631/326/417; 692/699/255/1629; Animal biology; Animals; Anopheles; Anopheles - microbiology; Burkina Faso; Ecology, environment; Female; Gram-Negative Bacterial Infections; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - transmission; Humanities and Social Sciences; Life Sciences; Male; Mosquito Control; multidisciplinary; Phylogeny; RNA, Ribosomal, 16S; RNA, Ribosomal, 16S - genetics; Science; Science (multidisciplinary); Symbiosis; Veterinary medicine and animal Health; Wolbachia; Wolbachia - genetics; Wolbachia - isolation & purification
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms4985

Wolbachia are maternally transmitted intracellular bacteria that invade insect populations by manipulating their reproduction and immunity and thus limiting the spread of numerous human pathogens. Experimental Wolbachia infections can reduce Plasmodium numbers in Anopheles mosquitoes in the laboratory, however, natural Wolbachia infections in field anophelines have never been reported. Here we show evidence of Wolbachia infections in Anopheles gambiae in Burkina Faso, West Africa. Sequencing of the 16S rRNA gene identified Wolbachia sequences in both female and male germlines across two seasons, and determined that these sequences are vertically transmitted from mother to offspring. Whole-genome sequencing of positive samples suggests that the genetic material identified in An. gambiae belongs to a novel Wolbachia strain, related to but distinct from strains infecting other arthropods. The evidence of Wolbachia infections in natural Anopheles populations promotes further investigations on the possible use of natural Wolbachia – Anopheles associations to limit malaria transmission. Wolbachia bacteria live within the cells of many insect species, manipulating their hosts’ reproduction and immune responses. Here, the authors show that these microbes also infect wild populations of malaria-spreading Anopheles mosquitoes, supporting a potential use of Wolbachia to limit malaria transmission.