Production of bone marrow, liver, thymus (BLT) humanized mice on the C57BL/6 Rag2−/−γc−/−CD47−/− background

C57BL/6 Rag2−/−γc−/−CD47−/− triple-knockout mice engrafted with fetal human bone marrow, liver, thymus (TKO-BLT) not only develop high levels of multi-lineage hematopoiesis but also organized lymphoid tissues including mesenteric lymph nodes, splenic follicles and gut-associated lymphoid tissues of...

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Bibliographic Details
Published in:Journal of immunological methods 2014-05, Vol.407, p.127-134
Main Authors: Lavender, Kerry J., Messer, Ronald J., Race, Brent, Hasenkrug, Kim J.
Format: Article
Language:English
Subjects:
Animals
BLT humanized mice
Bone Marrow - immunology
C57BL/6 Rag2−/−γc−/−CD47
DNA-Binding Proteins - genetics
Gene Knockout Techniques - methods
Graft vs Host Disease - immunology
Hematopoiesis
Humans
Interleukin Receptor Common gamma Subunit - genetics
Liver - immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Nuclear Proteins - genetics
Thymus Gland - immunology
Time Factors
Transplantation, Heterologous
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Summary:C57BL/6 Rag2−/−γc−/−CD47−/− triple-knockout mice engrafted with fetal human bone marrow, liver, thymus (TKO-BLT) not only develop high levels of multi-lineage hematopoiesis but also organized lymphoid tissues including mesenteric lymph nodes, splenic follicles and gut-associated lymphoid tissues of human origin. A unique advantage of these mice is that they sustain human cell and tissue engraftment long-term without the development of graft versus host disease. Thus they can be used for long-term studies not previously feasible with other models. The production of TKO-BLT mice to obtain healthy mice with high level reconstitution of human cells and tissues requires specialized methods that are presented in detail.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2014.04.008