Immunohistochemical analysis of p53, cyclinD1, RB1, c-fos and N-ras gene expression in hepatocellular carcinoma in Iran

• Published in: World journal of gastroenterology : WJG 2007-01, Vol.13 (4), p.588-593
• Main Authors: Moghaddam, S J, Haghighi, E N, Samiee, S, Shahid, N, Keramati, A R, Dadgar, S, Zali, M R
• Format: Article
• Language: English
• Subjects: Adult; Aged; Carcinoma, Hepatocellular - chemistry; Carcinoma, Hepatocellular - etiology; Carcinoma, Hepatocellular - genetics; Cyclin D1 - analysis; Female; Genes, ras; Humans; Immunohistochemistry; Liver Neoplasms - chemistry; Liver Neoplasms - etiology; Liver Neoplasms - genetics; Male; Middle Aged; Proto-Oncogene Proteins c-fos - analysis; Rapid Communication; Retinoblastoma Protein - analysis; Tumor Suppressor Protein p53 - analysis; 免疫组织化学; 组织病理学
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v13.i4.588

AIM： TO study the effect of some genes especially those involved in cell cycle regulation on hepatocellular carcinoma. METHODS： Paraffin-embedded tissue samples of 25 patients （18 males and 7 females） with hepatocellular carcinoma were collected from 22 pathology centers in Tehran during 2000-2001, and stained using immunohistochemistry method （avidin-biotin-peroxidase） for detection of p53, cyclinD1, RB1, c-los and N-ras proteins. RESULTS： Six （24%）, 5 （20%）, 12 （48%） and 2 samples （8%） were positive for p53, cyclinDl, C-los and N-ras expression, respectively. Twenty-two （88%） samples had alterations in the （31 cell-cycle checkpoint protein expression （RBI or cyclinD1）. P53 positive samples showed a higher （9 times） risk of being positive for RBI protein than p53 negative samples. Loss of expression of RBI in association with p53 over-expression was observed in 4 （66.7%） of 6 samples. Loss of expression of RBI was seen in all cyclinD1 positive, 20 （90.9%） N-ras negative, and ii （50%） C-fos positive samples, respectively. CyclinD1 positive samples showed a higher （2.85 and 4.75 times） risk of being positive for c-los and N-ras expression than cyclinD1 negative samples. CONCLUSION： The expression of p53, RB1 and c-los genes appears to have a key role in the pathogenesis of hepatocellular carcinoma in Iran. Simultaneous overexpression of these genes is significantly associated with their loss of expression during development of hepatocellular carcinoma.