Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin

• Published in: Scientific reports 2014-06, Vol.4 (1), p.5395-5395, Article 5395
• Main Authors: Yuan, Yuan, Jiang, Chao, Liu, Libing, Yu, Shulin, Cui, Zhanhu, Chen, Min, Lin, Shufang, Wang, Shu, Huang, Luqi
• Format: Article
• Language: English
• Subjects: 45; 45/47; 45/71; 49; 639/638/440/56; 692/53; 9/10; Buds; Chemical composition; Chromatography; DNA Mutational Analysis - methods; DNA polymerase; DNA, Plant - genetics; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - classification; Flowers & plants; Genotype; Genotype & phenotype; Genotyping; High-Throughput Nucleotide Sequencing - methods; High-throughput screening; Humanities and Social Sciences; Laboratories; Morphology; multidisciplinary; Nucleic Acid Amplification Techniques - methods; Plant species; Plants, Medicinal - genetics; Polymers; Polymorphism, Single Nucleotide - genetics; Reproducibility of Results; Science; Sensitivity and Specificity; Sequence Analysis, DNA - methods; Single-nucleotide polymorphism; Standard deviation; Values
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep05395

In this work, a rapid (within 4–5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials and is beneficial for detecting safety and quality of botanic products.