Experimental Protection of Diabetic Mice against Lethal P. aeruginosa Infection by Bacteriophage

The emergence of antibiotic-resistant bacterial strains has become a global crisis and is vulnerable for the exploration of alternative antibacterial therapies. The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa. P. aeruginosa was used to ind...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-11
Main Authors: Shivshetty, Nagaveni, Hosamani, Rajeshwari, Ahmed, Liyakat, Oli, Ajay Kumar, Sannauallah, Syed, Sharanbassappa, Shivshetty, Patil, S. A., Kelmani, Chandrakanth R.
Format: Article
Language:English
Subjects:
Animals
Antibacterial agents
Antibiotics
Bacteria
Bacteriology
Bacteriophages
Bacteriophages - genetics
Bacteriophages - pathogenicity
Communicable diseases
Diabetes
Diabetes Mellitus, Experimental - pathology
Diabetes Mellitus, Experimental - therapy
Diabetes Mellitus, Experimental - virology
Drug Resistance, Bacterial - genetics
Humans
Mice
Mice, Inbred NOD
Mortality
Nosocomial infections
Polyethylene glycol
Pseudomonas aeruginosa
Pseudomonas aeruginosa - pathogenicity
Pseudomonas aeruginosa - virology
Sepsis - microbiology
Sepsis - pathology
Sepsis - therapy
Sepsis - virology
Transmission electron microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The emergence of antibiotic-resistant bacterial strains has become a global crisis and is vulnerable for the exploration of alternative antibacterial therapies. The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa. P. aeruginosa was used to induce septicemia in streptozotocin (STZ) induced diabetic and nondiabetic mice by intraperitoneal (i.p.) injection of 3 × 108 CFU, resulting in a fatal bacteremia within 48 hrs. A single i.p. injection of 3 × 109 PFU phage GNCP showed efficient protection in both diabetic (90%) and nondiabetic (100%) bacteremic mice. It was further noted that the protection rate was reduced in diabetic mice when phage GNCP was administered after 4 h and 6 h of lethal bacterial challenge. In contrast, nondiabetic bacteremic mice were rescued even when treatment was delayed up to 20 h after lethal bacterial challenge. Evaluation of results confirmed that a single intraperitoneal injection of the phage dose (3 × 109 PFU/mL) was more effective than the multiple doses of imipenem. These results uphold the efficacy of phage therapy against pernicious P. aeruginosa infections, especially in cases of immunocompromised host.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/793242