Efficacy of Annona squamosa L in the Synthesis of Glycosaminoglycans and Collagen during Wound Repair in Streptozotocin Induced Diabetic Rats

The aim of this work was to find out the effects of Annona squamosa on the formation of glycosaminoglycans and collagen during wound healing in normal and diabetic rats. Diabetes induced rats were segregated into 4 groups, each containing six animals. Groups I and III served as the normal and diabet...

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Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-10
Main Authors: Ponrasu, Thangavel, Suguna, Lonchin
Format: Article
Language:English
Subjects:
Acids
Aldehydes - metabolism
Animals
Annona - chemistry
Annona squamosa
Care and treatment
Causes of
Cell adhesion & migration
Cell growth
Cell Proliferation - drug effects
Cell Survival - drug effects
Collagen
Collagen - biosynthesis
Complications and side effects
Dermis - pathology
Diabetes
Diabetes Mellitus, Experimental - chemically induced
Diabetes Mellitus, Experimental - drug therapy
Diabetes Mellitus, Experimental - pathology
Electrophoresis, Polyacrylamide Gel
Extracellular matrix
Fibroblasts - drug effects
Fibroblasts - pathology
Glycosaminoglycans
Glycosaminoglycans - biosynthesis
Health aspects
Humans
Industrial research
Magnoliidae
Male
Medicinal plants
Methods
Patient outcomes
Phytotherapy
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Rats, Wistar
Rodents
Scholarships & fellowships
Streptozocin
Treatment Outcome
Wound healing
Wound Healing - drug effects
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Summary:The aim of this work was to find out the effects of Annona squamosa on the formation of glycosaminoglycans and collagen during wound healing in normal and diabetic rats. Diabetes induced rats were segregated into 4 groups, each containing six animals. Groups I and III served as the normal and diabetic control while groups II and IV served as normal and diabetic treated. The animals were treated with 200 μL of Annona squamosa extract topically. The granulation tissues formed were removed on the 8th day and the amount of glycosaminoglycans (GAGs) and collagen formed was evaluated by sequential extraction and SDSPAGE, respectively. Histological evaluation was also carried out using Masson's trichrome stain. In vitro wound healing efficacy of A. squamosa in human dermal fibroblast culture (HDF) was also carried out. The fibroblasts treated with varying concentrations of A. squamosa were examined for proliferation and closure of the wound area and photographed. A. squamosa increased cellular proliferation in HDF culture. The granulation tissues of treated wounds showed increased levels of glycosaminoglycans (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/124352