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Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications
•Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS and peptide HDX-MS.•Applications: epitope mapping and comparability study. The higher order structure of protein therapeutics can be interro...
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| Published in: | Drug discovery today 2014-01, Vol.19 (1), p.95-102 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c463t-d1d8eb9ac9348e129e2c9cc16f39ac33e617423bb5795017f9e50e76faec9a9d3 |
| container_end_page | 102 |
| container_issue | 1 |
| container_start_page | 95 |
| container_title | Drug discovery today |
| container_volume | 19 |
| creator | Wei, Hui Mo, Jingjie Tao, Li Russell, Reb J. Tymiak, Adrienne A. Chen, Guodong Iacob, Roxana E. Engen, John R. |
| description | •Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS and peptide HDX-MS.•Applications: epitope mapping and comparability study.
The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described. |
| doi_str_mv | 10.1016/j.drudis.2013.07.019 |
| format | article |
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The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described.</description><subject>Animals</subject><subject>Deuterium - chemistry</subject><subject>Humans</subject><subject>Hydrogen - chemistry</subject><subject>Mass Spectrometry - methods</subject><subject>Protein Binding - physiology</subject><subject>Protein Interaction Maps - physiology</subject><subject>Protein Structure, Secondary</subject><subject>Protein Transport - physiology</subject><issn>1359-6446</issn><issn>1878-5832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kctu1TAQhi0EoqXwBgj5BZLacW5mgYQqepEqdVPWlmNPEh-d2NHYqTgvwHPXRwcKbNjY1sz833jmJ-QjZyVnvL3clRY362JZMS5K1pWMy1fknPddXzS9qF7nt2hk0dZ1e0bexbhjjFeyad-Ss0rIqmeyOyc_bw8WwwT-0sKWAN22UPhhZu0noIuOkcYVTMKwQMIDHQPSFcPg_ERnN82ANKDNZ0y4mbQh0DAeKxI4T1PO6zVznYmfaSbMwYZ9mA5Ue0v1uu6d0ckFH9-TN6PeR_jw674g36-_PV7dFvcPN3dXX-8LU7ciFZbbHgapjRR1D3kaqIw0hrejyEEhoOVdXYlhaDrZMN6NEhoGXTtqMFJLKy7IlxN33YYFrAGfUO_Vim7ReFBBO_VvxrtZTeFJ1azndV9lQH0CGAwxIowvWs7U0Re1Uydf1NEXxTqVfcmyT3_3fRH9NuLPxyBP_-QAVTQOvAHrMO9f2eD-3-EZDpWntA</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Wei, Hui</creator><creator>Mo, Jingjie</creator><creator>Tao, Li</creator><creator>Russell, Reb J.</creator><creator>Tymiak, Adrienne A.</creator><creator>Chen, Guodong</creator><creator>Iacob, Roxana E.</creator><creator>Engen, John R.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications</title><author>Wei, Hui ; Mo, Jingjie ; Tao, Li ; Russell, Reb J. ; Tymiak, Adrienne A. ; Chen, Guodong ; Iacob, Roxana E. ; Engen, John R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-d1d8eb9ac9348e129e2c9cc16f39ac33e617423bb5795017f9e50e76faec9a9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Deuterium - chemistry</topic><topic>Humans</topic><topic>Hydrogen - chemistry</topic><topic>Mass Spectrometry - methods</topic><topic>Protein Binding - physiology</topic><topic>Protein Interaction Maps - physiology</topic><topic>Protein Structure, Secondary</topic><topic>Protein Transport - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Hui</creatorcontrib><creatorcontrib>Mo, Jingjie</creatorcontrib><creatorcontrib>Tao, Li</creatorcontrib><creatorcontrib>Russell, Reb J.</creatorcontrib><creatorcontrib>Tymiak, Adrienne A.</creatorcontrib><creatorcontrib>Chen, Guodong</creatorcontrib><creatorcontrib>Iacob, Roxana E.</creatorcontrib><creatorcontrib>Engen, John R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Drug discovery today</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Hui</au><au>Mo, Jingjie</au><au>Tao, Li</au><au>Russell, Reb J.</au><au>Tymiak, Adrienne A.</au><au>Chen, Guodong</au><au>Iacob, Roxana E.</au><au>Engen, John R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications</atitle><jtitle>Drug discovery today</jtitle><addtitle>Drug Discov Today</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>19</volume><issue>1</issue><spage>95</spage><epage>102</epage><pages>95-102</pages><issn>1359-6446</issn><eissn>1878-5832</eissn><abstract>•Hydrogen/deuterium exchange mass spectrometry (HDX-MS) technology for assessment of higher order structure of protein therapeutics.•Methodology: global HDX-MS and peptide HDX-MS.•Applications: epitope mapping and comparability study.
The higher order structure of protein therapeutics can be interrogated with hydrogen/deuterium exchange mass spectrometry (HDX-MS). HDX-MS is now a widely used tool in the structural characterization of protein therapeutics. In this review, HDX-MS based workflows designed for protein therapeutic discovery and development processes are presented, focusing on the specific applications of epitope mapping for protein/drug interactions and biopharmaceutical comparability studies. Future trends in the application of HDX-MS in protein therapeutics characterization are also described.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23928097</pmid><doi>10.1016/j.drudis.2013.07.019</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-6446 |
| ispartof | Drug discovery today, 2014-01, Vol.19 (1), p.95-102 |
| issn | 1359-6446 1878-5832 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4081482 |
| source | ScienceDirect Journals |
| subjects | Animals Deuterium - chemistry Humans Hydrogen - chemistry Mass Spectrometry - methods Protein Binding - physiology Protein Interaction Maps - physiology Protein Structure, Secondary Protein Transport - physiology |
| title | Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications |
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