Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1)

• Published in: Scientific reports 2014-07, Vol.4 (1), p.5597-5597, Article 5597
• Main Authors: Broeckx, Bart J. G., Coopman, Frank, Verhoeven, Geert E. C., Bavegems, Valérie, De Keulenaer, Sarah, De Meester, Ellen, Van Niewerburgh, Filip, Deforce, Dieter
• Format: Article
• Language: English
• Subjects: 45; 45/23; 49; 631/61/212/2301; 631/61/514/2256; 692/308/2056; Amino acid sequence; Animals; Dogs; Exome; Exons; Genomes; High-Throughput Nucleotide Sequencing; Humanities and Social Sciences; multidisciplinary; Reproducibility of Results; Science; Sensitivity and Specificity; Sequence Analysis, DNA
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep05597

Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum coverage of five and 90% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies.