Analytical characteristics of a qPCR-based molecular diagnostic assay-conceptual considerations for laboratory personnel

Dear Editor： Quantitative real-time PCR has revolutionized molecular diagnostics with its ease of use,increased sensitivity and specificity and low turnaround time.PCR/quantitative PCR（qPCR）-based assays offer a distinct advantage over other serological/conventional diagnostic approaches.The ability...

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Bibliographic Details
Published in:Journal of biomedical research 2014-01, Vol.28 (3), p.246-248
Main Authors: Shukla, Tanuj, Kaipa, Prabhakar Rao
Format: Article
Language:English
Subjects:
Letter to the Editor
世界卫生组织
分子诊断
基础
定量PCR
实验人员
概念性
欧洲标准
诊断检测
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Summary:Dear Editor： Quantitative real-time PCR has revolutionized molecular diagnostics with its ease of use,increased sensitivity and specificity and low turnaround time.PCR/quantitative PCR（qPCR）-based assays offer a distinct advantage over other serological/conventional diagnostic approaches.The ability to diagnose infectious diseases has benefited from the availability of US FDA approved and Conformite Europeenne（CE）-marked qPCR-based in-vitro diagnostic kits from international companies.The high-quality kits are calibrated with the World Health Organization（WHO）reference standards and the National Institute for Biological Standards and Control（NIBSC）standards.
ISSN:1674-8301
DOI:10.7555/JBR.28.20140046