Loss of heterozygosity and microsatellite instabilities of fragile histidine triad gene in gastric carcinoma

AIM： To detect the loss of heterozygosity （LOH） and microsatellite instabilities （MSI） of fragile histidine triad （FHIT） gene in gastric carcinoma and to study their association with the clinical pathological characteristics of gastric carcinoma. METHODS： LOH and MSI of FHIT gene were detected at fo...

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Published in:World journal of gastroenterology : WJG 2006-06, Vol.12 (23), p.3766-3769
Main Authors: Xiao, Yu-Ping, Wu, Dong-Ying, Xu, Lei, Xin, Yan
Format: Article
Language:English
Subjects:
Acid Anhydride Hydrolases - genetics
Adenocarcinoma - genetics
DNA, Neoplasm - genetics
Gene Expression Regulation, Neoplastic
Genomic Instability
Humans
Loss of Heterozygosity
Lymphatic Metastasis - genetics
Microsatellite Repeats - genetics
Neoplasm Proteins - genetics
Rapid Communication
Stomach Neoplasms - genetics
基因表达
病理机制
组氨酸
胃肿瘤
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Summary:AIM： To detect the loss of heterozygosity （LOH） and microsatellite instabilities （MSI） of fragile histidine triad （FHIT） gene in gastric carcinoma and to study their association with the clinical pathological characteristics of gastric carcinoma. METHODS： LOH and MSI of FHIT gene were detected at four microsaterllite loci D3SI3H, D3S4103, D3SI481 and D3S1234 using PCR in matched normal and cancerous tissues from 50 patients with primary gastric cancer. RESULTS： The average frequency of LOH and MSI of FHIT gene in gastric cancer was 32.4% and 26.4% respectively. LOH and MSI of FHIT gene in gastric cancer had no association with histological, Borrmann, and Lauren＇s classification. LOH of FHIT gene in gastric cancer was related to invasive depth. The frequency of FHIT LOH in gastric cancer with serosa-penetration was obviously higher than that in gastric cancer without serosa-penetration （73.5% vs 37.5%, P 〈 0.05）. MSI of FHIT gene in gastric cancer was associated with the lymph node metastasis. The frequency of MSI in gastric cancer without lymph node metastasis was significantly higher than that in gastric cancer with lymph node metastasis （66.7% vs 34.3%, P 〈 0.05）. CONCLUSION： LOH of FHIT gene is correlated with invasive depth of gastric carcinoma. MSI of FHIT gene is correlated with lymph node metastases. LOH and MSI of FHIT gene play an important role in carcinogenesis of gastric cancer.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v12.i23.3766