Oxygen radical formation does not have an impact in the treatment of severe acute experimental pancreatitis using free cellular hemoglobin

• Published in: World journal of gastroenterology : WJG 2006-05, Vol.12 (18), p.2914-2918
• Main Authors: Kleinhans, Helge, Mann, Oliver, Schurr, Paulus G, Kaifi, Jussuf T, Hansen, Bente, Izbicki, Jakob R, Strate, Tim
• Format: Article
• Language: English
• Subjects: Acute Disease; Animals; Disease Models, Animal; Female; Hemoglobins - pharmacology; Hemoglobins - therapeutic use; Hydroxyethyl Starch Derivatives - pharmacology; Hydroxyethyl Starch Derivatives - therapeutic use; Lipid Peroxidation - drug effects; Lipid Peroxidation - physiology; Microcirculation - physiology; Pancreas - blood supply; Pancreas - metabolism; Pancreas - pathology; Pancreas - physiopathology; Pancreatitis - drug therapy; Pancreatitis - pathology; Pancreatitis - physiopathology; Plasma Substitutes - pharmacology; Plasma Substitutes - therapeutic use; Rapid Communication; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Regional Blood Flow - physiology; 实验研究; 病理机制; 胰腺炎; 血红蛋白
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i18.2914

AIM： Microcirculatory dysfunction and free oxygen radicals are important factors in the pathogenesis of severe acute pancreatitis. Additional oxygen delivery might enhance lipid peroxidation but may also improve pancreatic microcirculation. This study assesses the effect of free cellular bovine hemoglobin on the formation of oxygen radicals and microcirculation in a rodent model of severe acute pancreatitis. METHODS： Fifteen minutes after induction of acute pancreatitis Wistar rats received either 0.8 mL bovine hemoglobin （HBOC-200）, hydroxyethyl starch （HES） or 2.4 mL of normal saline to ensure normovolemic substitution. After 6 h of examination the pancreas was excised and rapidly processed for indirect measurement of lipid peroxidation products malondialdehyde （MDA） and reduced glutathione （GSH） in pancreatic tissue. RESULTS： The single application of HBOC-200 improved pancreatic microcirculation and reduced histopathological tissue damage significantly. Tissue concentration of MDA did not differ between the groups. Also no differences in GSH levels were detected. CONCLUSION： Though the single application of HBOC-200 and HES improve pancreatic microcirculation, no differences in lipid peroxidation products were detected. The beneficial effect of additional oxygen supply （HBOC-200） does not lead to enhanced lipid peroxidation.