Eubacterium limosum ameliorates experimental colitis and metabolite of microbe attenuates colonic inflammatory action with increase of mucosal integrity

• Published in: World journal of gastroenterology : WJG 2006-02, Vol.12 (7), p.1071-1077
• Main Authors: Kanauchi, Osamu, Fukuda, Masanobu, Matsumoto, Yoshiaki, Ishii, Shino, Ozawa, Toyokazu, Shimizu, Makiko, Mitsuyama, Keiichi, Andoh, Akira
• Format: Article
• Language: English
• Subjects: Acetates - metabolism; Acetates - pharmacology; Animals; Basic Research; Butyrates - metabolism; Butyrates - pharmacology; Cell Line; Cell Proliferation; Colitis - metabolism; Colitis - pathology; Colitis - physiopathology; Colitis - therapy; Colon - chemistry; Colon - microbiology; Colon - pathology; Colon - physiopathology; Eubacterium - metabolism; Eubacterium - physiology; Fatty Acids, Volatile - metabolism; Fatty Acids, Volatile - physiology; Female; Gene Expression Regulation; Humans; Inflammatory Bowel Diseases - metabolism; Inflammatory Bowel Diseases - pathology; Inflammatory Bowel Diseases - physiopathology; Inflammatory Bowel Diseases - therapy; Interleukin-6 - metabolism; Intestinal Mucosa - chemistry; Intestinal Mucosa - microbiology; Intestinal Mucosa - pathology; Intestinal Mucosa - physiopathology; Mice; Mice, Inbred BALB C; Propionates - metabolism; Propionates - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Messenger - genetics; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - physiology; Tumor Necrosis Factor-alpha - pharmacology; 实验研究; 微生物; 结肠炎
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i7.1071

AIM： To examine the effect of Eubacteriuro Iiroosuro （E.Iiroosuro） on colonic epithelial cell line in vitro, and to evaluate the effect of E.limosum on experimental colitis. METHODS： E.Iimosum was inoculated anaerobically and its metabolites were obtained. The growth stimulatory effect of the E.limosum metabolites on T84 cells was evaluated by SUDH activity, and the anti-inflammatory effect by IL-6 production. The change in mRNA of toll like receptor 4 （TLR4） was evaluated by real time PCR. Colitis was induced by feeding BALB/C mice with 2.0% dextran sodium sulfate. These mice received either 5% lyophilized E.lirnosum （n=7） or control diet （n=7）. Seven days after colitis induction, clinical and histological scores, colon length, and cecal organic acid levels were determined. RESULTS： The E.Iimosum produced butyrate, acetate, propionate, and lactate at 0.25, 1.0, 0.025 and 0.07 retool/L, respectively in medium. At this concentration, each acid had no growth stimulating activity on T84 cells; however, when these acids were mixed together at the above levels, it showed significantly high activity than control. Except for lactate, these acids significantly attenuated IL-6 production at just 0.1 mmol/L. In addition, under TNF-α stimulation, butyrate attenuated the production of TLR4 mRNA. The treatment with E.limosum significantly attenuated clinical and histological scores of colitis with an increase of cecal butyrate levels, compared with the control group. CONCLUSION： E.limosum can ameliorate experimental colonic inflammation. In part, the metabolite of E.lirnosurn, butyrate, increases mucosal integrity and shows anti-inflammatory action modulation of mucosal defense system via TLR4.