In vitro porcine brain tubulin assembly inhibition by water extract from a Chinese medicinal herb, Tripterygium hypoglaucum Hutch

AIM： To investigate the effect of Tripterygium hyp-oglaucum Hutch （THH） on the assembly and disassembly process of tubulin and its possible mode of action. METHODS： In vitro porcine brain tubulin assembly assay was employed to analyze the inhibitory effects of THH at different concentrations （0.05 μ...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2006-02, Vol.12 (7), p.1133-1135
Main Authors: Liang, Zi-Qin, Cao, Neng, Song, Zhong-Kui, Wang, Xu
Format: Article
Language:English
Subjects:
Aneugens - adverse effects
Aneugens - pharmacology
Aneuploidy
Animals
Brain - metabolism
Brain Chemistry - drug effects
Dose-Response Relationship, Drug
Drugs, Chinese Herbal - adverse effects
Drugs, Chinese Herbal - pharmacology
In Vitro Techniques
Phytotherapy - adverse effects
Rapid Communication
Risk
Swine
Tripterygium - chemistry
Tubulin - metabolism
中医药疗法
微管蛋白
抑制作用
脑疾病
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Summary:AIM： To investigate the effect of Tripterygium hyp-oglaucum Hutch （THH） on the assembly and disassembly process of tubulin and its possible mode of action. METHODS： In vitro porcine brain tubulin assembly assay was employed to analyze the inhibitory effects of THH at different concentrations （0.05 μg/L, 0.07 μg/L, 0.09μg/L）. Colchicine （0.0025 mmol/L, 0.0050 mmol/L, 0.0075 mmol/L） was used as a positive control. RESULTS： THH could significantly inhibit the assembly of isolated porcine brain tubulin at all tested concentrations. CONCLUSION： THH is capable of inducing aneuploidy in mammals via tubulin polymerization inhibition pathway and may pose a genetic risk to human beings.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v12.i7.1133