Increased expression of angiogenin in gastric carcinoma in correlation with tumor angiogenesis and proliferation

• Published in: World journal of gastroenterology : WJG 2006-08, Vol.12 (32), p.5135-5139
• Main Authors: Chen, Yu, Zhang, Sheng, Chen, Yu-Peng, Lin, Jian-Yin
• Format: Article
• Language: English
• Subjects: Aged; Cell Proliferation; Female; Gastric Cancer; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Ki-67 Antigen - biosynthesis; Male; Microcirculation; Middle Aged; Neovascularization, Pathologic; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease, Pancreatic - biosynthesis; Ribonuclease, Pancreatic - physiology; RNA, Messenger - metabolism; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Vascular Endothelial Growth Factor A - biosynthesis; 肿瘤血管生成; 血管内皮生长因子; 血管生成素
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i32.5135

AIM： To investigate the implication of angiogenin （ANG） in the neovascularizaton and growth of human gastric carcinoma （HGC）. METHODS： ANG mRNA expression in HGC specimens obtained by surgical resection from patients with HGC were examined by RT-PCR. ANG, Ki-67, VEGF protein expression and microvessel density （MVD） in HGC specimens were detected by immunohistochemistry. RESULTS： RT-PCR showed significantly higher ANG mRNA expression （0.482 ± 0.094） in HGC tissues than in the surrounding nontumorous tissues （0.276 ±0.019, P = 0.03）. MVD within tumorous tissues increased significantly with ANG mRNA expression （r = 0.380, P = 0.001） and ANG protein expression （P 〈 0.01）. The ANG expression levels of cancer tissues were positively correlated with VEGF （P 〈 0.01） and the proliferation index of cancer cells （P 〈 0.01）. CONCLUSION： ANG is one of the neovascularization factors of HGC. ANG may work in coordination with VEGF, and promote the proliferation of HGC cells.