K-19 mRNA RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer

AIM： To investigate the value and prospect of RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer. METHODS： Histopathology was used and K19 mRNA expression was detected by RT-PCR in tumor tissues and lymph nodes from gastric cancer patients undergoing radical resection of g...

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Published in:World journal of gastroenterology : WJG 2006-08, Vol.12 (32), p.5219-5222
Main Authors: Suo, Jian, Wang, Quan, Jin, Hong-Juan, Li, Hong, Zhao, Hang
Format: Article
Language:English
Subjects:
Actins - genetics
Biomarkers, Tumor
Carcinoma - pathology
DNA Primers - chemistry
Humans
Keratin-19 - genetics
Lymphatic Metastasis
Neoplasm Metastasis
Prognosis
Rapid Communication
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
淋巴结
肿瘤转移
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Summary:AIM： To investigate the value and prospect of RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer. METHODS： Histopathology was used and K19 mRNA expression was detected by RT-PCR in tumor tissues and lymph nodes from gastric cancer patients undergoing radical resection of gastric carcinoma. RESULTS： K19 mRNA was expressed in all tumor specimens of 30 cases; of the 126 lymph nodes, 26 were histopathologically positive （20.6%）, and 42 positive （33.3%） by RT-PCR. Amplification fragments of 460 and 540 bp were shown in all the tumor tissues and metastatic lymph nodes after K19 and β-actin RT-PCR, while only a 540 bp fragment appeared in the lymph nodes of non-tumor patients. CONCLUSION： K19 mRNA RT-PCR is sensitive and specific in testing micrometastasis in regional lymph nodes of gastric cancer, and it is superior to routine histopathology.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v12.i32.5219