Detection of H. pylori infection by ELISA and Western blot techniques and evaluation of anti CagA seropositivity in adult Turkish dyspeptic patients

To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients. We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay (ELISA) and Western blot in dyspeptic Turkish patients. H pylori status was determine...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2006-09, Vol.12 (33), p.5375-5378
Main Authors: Yilmaz, Ozlem, Sen, Nazime, Küpelioğlu, Ahmet Ali, Simşek, Ilkay
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens, Bacterial - blood
Antigens, Bacterial - genetics
Bacterial Proteins - blood
Bacterial Proteins - genetics
Biopsy
Blotting, Western - methods
Dyspepsia - blood
Dyspepsia - microbiology
Enzyme-Linked Immunosorbent Assay - methods
Female
Helicobacter Infections - blood
Helicobacter Infections - diagnosis
Helicobacter Infections - microbiology
Humans
Male
Middle Aged
Rapid Communication
Reproducibility of Results
Turkey
Virulence Factors
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Summary:To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients. We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay (ELISA) and Western blot in dyspeptic Turkish patients. H pylori status was determined by histology and rapid urease testing. Fifty-six patients were entered. Forty-eight (85.7%) out of the 56 patients were positive for H pylori. H pylori IgG seropositivity was 82.1%, IgA seropositivity 48.2%. CagA ELISA showed that IgG was positive in 50% and IgA in 30.4% of those with H pylori infections. Western blot showed that IgG seropositivity was 80.4% and IgA seropositivity 33.9%. Western blot detected IgG antibodies with reactivity to CagA in 50%, VacA in 62.5%, UreB in 87.5%, UreA in 80.4%, and OMP in 57.1%. None of the tests had a sensitivity and specificity above 80%. None of these commercial tests seems clinically useful for H pylori detection in adult dyspeptic patients, while Western blot can give seropositivity and determine anti-CagA, VacA virulence factor status of Turkish dyspeptic patients in the Izmir region.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v12.i33.5375