Adenylyl Cyclase Signaling in the Developing Chick Heart : The Deranging Effect of Antiarrhythmic Drugs

The adenylyl cyclase (AC) signaling system plays a crucial role in the regulation of cardiac contractility. Here we analyzed the key components of myocardial AC signaling in the developing chick embryo and assessed the impact of selected β-blocking agents on this system. Application of metoprolol an...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-6
Main Authors: Hejnova, Lucie, Hahnova, Klara, Kockova, Radka, Svatunkova, Jarmila, Sedmera, David, Novotny, Jiri
Format: Article
Language:English
Subjects:
Adenylate cyclase
Adenylyl Cyclases - biosynthesis
Adenylyl Cyclases - genetics
Anatomy & physiology
Animals
Anti-Arrhythmia Agents - administration & dosage
Anti-arrhythmia drugs
Antiarrhythmics
Biomedical research
Carbazoles - administration & dosage
Cardiovascular diseases
Chick Embryo
Chickens - genetics
Chickens - growth & development
Drug therapy
Eggs
Gene Expression Regulation - drug effects
Genetic aspects
Health aspects
Heart
Heart - drug effects
Heart - growth & development
Heart rate
Metoprolol - administration & dosage
Myocardium - enzymology
Physiological aspects
Postpartum period
Pregnancy
Propanolamines - administration & dosage
Proteins
Rodents
Signal Transduction - drug effects
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Summary:The adenylyl cyclase (AC) signaling system plays a crucial role in the regulation of cardiac contractility. Here we analyzed the key components of myocardial AC signaling in the developing chick embryo and assessed the impact of selected β-blocking agents on this system. Application of metoprolol and carvedilol, two commonly used β-blockers, at embryonic day (ED) 8 significantly downregulated (by about 40%) expression levels of AC5, the dominant cardiac AC isoform, and the amount of Gsα protein at ED9. Activity of AC stimulated by forskolin was also significantly reduced under these conditions. Interestingly, when administered at ED4, these drugs did not produce such profound changes in the myocardial AC signaling system, except for markedly increased expression of Giα protein. These data indicate that β-blocking agents can strongly derange AC signaling during the first half of embryonic heart development.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/463123