Antifungal activity of synthetic naphthoquinones against dermatophytes and opportunistic fungi: preliminary mechanism-of-action tests

This study evaluated the antifungal activities of synthetic naphthoquinones against opportunistic and dermatophytic fungi and their preliminary mechanisms of action. The minimum inhibitory concentrations (MICs) of four synthetic naphthoquinones for 89 microorganisms, including opportunistic yeast ag...

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Bibliographic Details
Published in:Annals of clinical microbiology and antimicrobials 2014-07, Vol.13 (1), p.26-26, Article 26
Main Authors: Ferreira, Maria do Perpetuo Socorro Borges Carriço, Cardoso, Mariana Filomena do Carmo, da Silva, Fernando de Carvalho, Ferreira, Vitor Francisco, Lima, Emerson Silva, Souza, João Vicente Braga
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Cell Membrane Permeability - drug effects
Cryptococcus
Fungal infections
Fungi
Fungi - drug effects
Fungi - isolation & purification
Furans
Humans
Microbial Sensitivity Tests
Microorganisms
Mycoses - microbiology
Naphthoquinones - pharmacology
Studies
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Summary:This study evaluated the antifungal activities of synthetic naphthoquinones against opportunistic and dermatophytic fungi and their preliminary mechanisms of action. The minimum inhibitory concentrations (MICs) of four synthetic naphthoquinones for 89 microorganisms, including opportunistic yeast agents, dermatophytes and opportunistic filamentous fungi, were determined. The compound that exhibited the best activity was assessed for its action against the cell wall (sorbitol test), for interference associated with ergosterol interaction, for osmotic balance (K+ efflux) and for membrane leakage of substances that absorb at the wavelength of 260 nm. All tested naphthoquinones exhibited antifungal activity, and compound IVS320 (3a,10b-dihydro-1H-cyclopenta [b] naphtho [2,3-d] furan-5,10-dione)-dione) demonstrated the lowest MICs across the tested species. The MIC of IVS320 was particularly low for dermatophytes (values ranging from 5-28 μg/mL) and Cryptococcus spp. (3-5 μg/mL). In preliminary mechanism-of-action tests, IVS320 did not alter the fungal cell wall but did cause problems in terms of cell membrane permeability (efflux of K+ and leakage of substances that absorb at 260 nm). This last effect was unrelated to ergosterol interactions with the membrane.
ISSN:1476-0711
1476-0711
DOI:10.1186/1476-0711-13-26