Molecular identification and interaction assay of the gene (OsUbc13) encoding a ubiquitin-conjugating enzyme in rice

The ubiquitin (Ub)-conjugating enzyme, Ubc13, has been known to be involved in error-free DNA damage tolerance (or post-replication repair) via catalyzing Lys63-linked polyubiquitin chains formation together with a Ubc variant. However, its functions remain largely unknown in plant species, especial...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2014-07, Vol.15 (7), p.624-637
Main Authors: Wang, Ya, Xu, Meng-yun, Liu, Jian-ping, Wang, Mu-gui, Yin, Hai-qing, Tu, Ju-min
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biomedical and Life Sciences
Biomedicine
Biomedicine & Biotechnology
Molecular Sequence Data
Nuclear Envelope - enzymology
Oryza - genetics
Oryza sativa
Review
Two-Hybrid System Techniques
Ubiquitin-Conjugating Enzymes - chemistry
Ubiquitin-Conjugating Enzymes - genetics
Ubiquitin-Conjugating Enzymes - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ubiquitin (Ub)-conjugating enzyme, Ubc13, has been known to be involved in error-free DNA damage tolerance (or post-replication repair) via catalyzing Lys63-linked polyubiquitin chains formation together with a Ubc variant. However, its functions remain largely unknown in plant species, especially in monocotyledons. In this study, we cloned a Ub-conjugating enzyme, OsUbc13, that shares the conserved domain of Ubc with AtUBC13B in Oryza sativa L., which encodes a protein of 153 amino acids; the deduced sequence shares high similarities with other homologs. Real-time quantitative polymerase chain reaction (PCR) indicated that OsUbc13 transcripts could be detected in all tissues examined, and the expression level was higher in palea, pistil, stamen, and leaf, and lower in root, stem, and lemma; the expression of OsUbc13 was induced by low temperature, methylmethane sulfate (MMS), and H 2 O 2 , but repressed by mannitol, abscisic acid (ABA), and NaCl. OsUbc13 was probably localized in the plasma and nuclear membranes. About 20 proteins, which are responsible for the positive yeast two-hybrid interaction of OsUbc13 , were identified. These include the confirmed OsVDAC (correlated with apoptosis), OsMADS1 (important for development of floral organs), OsB22EL8 (related to reactive oxygen species (ROS) scavenging and DNA protection), and OsCROC-1(required for formation of Lys63 polyubiquitylation and error-free DNA damage tolerance). The molecular characterizationprovides a foundation for the functional study of OsUbc13 .
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1300273