Quantitative differential expression analysis reveals miR-7 as major islet microRNA

MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miR...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-02, Vol.366 (4), p.922-926
Main Authors: Bravo-Egana, Valia, Rosero, Samuel, Molano, R. Damaris, Pileggi, Antonello, Ricordi, Camillo, Domínguez-Bendala, Juan, Pastori, Ricardo L.
Format: Article
Language:English
Subjects:
Animals
Gene Expression Profiling
Gene Expression Regulation
Humans
Islets of Langerhans - cytology
Islets of Langerhans - metabolism
MicroRNA microarrays
MicroRNAs
MicroRNAs - genetics
Oligonucleotide Array Sequence Analysis
Pancreatic islets
q-RT-PCR
Rats
Rats, Inbred Lew
Reverse Transcriptase Polymerase Chain Reaction
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Summary:MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.12.052