Evaluation of two molecular methods for the detection of Yellow fever virus genome

Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of tra...

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Bibliographic Details
Published in:Journal of virological methods 2011-06, Vol.174 (1-2), p.29-34
Main Authors: Nunes, Marcio R.T., Palacios, Gustavo, Nunes, Keley N.B., Casseb, Samir M.M., Martins, Lívia C., Quaresma, Juarez A.S., Savji, Nazir, Lipkin, W. Ian, Vasconcelos, Pedro F.C.
Format: Article
Language:English
Subjects:
acute course
Animals
arboviruses
Biological and medical sciences
blood serum
Cricetinae
diagnostic techniques
Flaviviridae
Flavivirus
Fundamental and applied biological sciences. Psychology
genome
Genomes
Humans
Mesocricetus
Mesocricetus auratus
Microbiology
molecular diagnostic
Molecular Diagnostic Techniques - methods
monitoring
Public health
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - methods
RT-hemi-nested-PCR
Serum - virology
South America
SYBR® Green qRT-PCR
Techniques used in virology
urban areas
Virology
Virology - methods
Yellow Fever - diagnosis
Yellow Fever - virology
Yellow fever virus
Yellow fever virus - genetics
Yellow fever virus - isolation & purification
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Description
Summary:Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR® Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2011.02.025