Expression of KOC, S100P, mesothelin and MUC1 in pancreatico-biliary adenocarcinomas: development and utility of a potential diagnostic immunohistochemistry panel

Pancreatico-biliary adenocarcinomas (PBA) have a poor prognosis. Diagnosis is usually achieved by imaging and/or endoscopy with confirmatory cytology. Cytological interpretation can be difficult especially in the setting of chronic pancreatitis/cholangitis. Immunohistochemistry (IHC) biomarkers coul...

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Bibliographic Details
Published in:BMC clinical pathology 2014-07, Vol.14 (1), p.35-35, Article 35
Main Authors: Ali, Asif, Brown, Victoria, Denley, Simon, Jamieson, Nigel B, Morton, Jennifer P, Nixon, Colin, Graham, Janet S, Sansom, Owen J, Carter, C Ross, McKay, Colin J, Duthie, Fraser R, Oien, Karin A
Format: Article
Language:English
Subjects:
Diagnosis
Immunohistochemistry
Medical research
Mucins
Pancreas
Pancreatic cancer
Pathology
Prognosis
Studies
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Summary:Pancreatico-biliary adenocarcinomas (PBA) have a poor prognosis. Diagnosis is usually achieved by imaging and/or endoscopy with confirmatory cytology. Cytological interpretation can be difficult especially in the setting of chronic pancreatitis/cholangitis. Immunohistochemistry (IHC) biomarkers could act as an adjunct to cytology to improve the diagnosis. Thus, we performed a meta-analysis and selected KOC, S100P, mesothelin and MUC1 for further validation in PBA resection specimens. Tissue microarrays containing tumour and normal cores in a ratio of 3:2, from 99 surgically resected PBA patients, were used for IHC. IHC was performed on an automated platform using antibodies against KOC, S100P, mesothelin and MUC1. Tissue cores were scored for staining intensity and proportion of tissue stained using a Histoscore method (range, 0-300). Sensitivity and specificity for individual biomarkers, as well as biomarker panels, were determined with different cut-offs for positivity and compared by summary receiver operating characteristic (ROC) curve. The expression of all four biomarkers was high in PBA versus normal ducts, with a mean Histoscore of 150 vs. 0.4 for KOC, 165 vs. 0.3 for S100P, 115 vs. 0.5 for mesothelin and 200 vs. 14 for MUC1 (p 
ISSN:1472-6890
1472-6890
DOI:10.1186/1472-6890-14-35