Optic nerve crush induces spatial and temporal gene expression patterns in retina and optic nerve of BALB/cJ mice

Central nervous system (CNS) trauma and neurodegenerative disorders trigger a cascade of cellular and molecular events resulting in neuronal apoptosis and regenerative failure. The pathogenic mechanisms and gene expression changes associated with these detrimental events can be effectively studied u...

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Bibliographic Details
Published in:Molecular neurodegeneration 2014-04, Vol.9 (1), p.14-14, Article 14
Main Authors: Sharma, Tasneem P, McDowell, Colleen M, Liu, Yang, Wagner, Alex H, Thole, David, Faga, Benjamin P, Wordinger, Robert J, Braun, Terry A, Clark, Abbot F
Format: Article
Language:English
Subjects:
Analysis
Animals
Apoptosis
Apoptosis - genetics
Bioinformatics
Biotechnology industry
Computer software industry
Disease Models, Animal
Gene expression
Gene Expression Regulation - genetics
Genetic aspects
Health aspects
Immunohistochemistry
Injuries
Mice
Mice, Inbred BALB C
Nerve Crush
Nerve Degeneration - genetics
Nerve Regeneration - genetics
Nervous system
Neurodegeneration
Neurodegenerative Diseases - genetics
Neurodegenerative Diseases - metabolism
Neurodegenerative Diseases - pathology
Neurons
Oligonucleotide Array Sequence Analysis
Optic nerve
Optic Nerve - metabolism
Optic Nerve - pathology
Optics
Proteins
Real-Time Polymerase Chain Reaction
Retina - metabolism
Retina - pathology
Reverse Transcriptase Polymerase Chain Reaction
Studies
Survival analysis
Transcriptome
Variance analysis
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Summary:Central nervous system (CNS) trauma and neurodegenerative disorders trigger a cascade of cellular and molecular events resulting in neuronal apoptosis and regenerative failure. The pathogenic mechanisms and gene expression changes associated with these detrimental events can be effectively studied using a rodent optic nerve crush (ONC) model. The purpose of this study was to use a mouse ONC model to: (a) evaluate changes in retina and optic nerve (ON) gene expression, (b) identify neurodegenerative pathogenic pathways and (c) discover potential new therapeutic targets. Only 54% of total neurons survived in the ganglion cell layer (GCL) 28 days post crush. Using Bayesian Estimation of Temporal Regulation (BETR) gene expression analysis, we identified significantly altered expression of 1,723 and 2,110 genes in the retina and ON, respectively. Meta-analysis of altered gene expression (≥1.5, ≤-1.5, p 
ISSN:1750-1326
1750-1326
DOI:10.1186/1750-1326-9-14