Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Uncomplexed Highly Sulfated Oligosaccharides Using Ionic Liquid Matrices

Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulf...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2006-03, Vol.78 (6), p.1774-1779
Main Authors: Laremore, Tatiana N, Murugesan, Saravanababu, Park, Tae-Joon, Avci, Fikri Y, Zagorevski, Dmitri V, Linhardt, Robert J
Format: Article
Language:English
Subjects:
Analytical chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Chemical bonds
Chemistry
Cinnamates - chemistry
Exact sciences and technology
Imidazoles - chemistry
Ionic Liquids - chemistry
Mass spectrometry
Molecular Sequence Data
Oligosaccharides - analysis
Sensitivity and Specificity
Spectrometric and optical methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Sulfates - chemistry
Sulfur content
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Summary:Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium α-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O−SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac051121q