Differential expression of transforming growth factor-beta in benign vs. papillary thyroid cancer nodules; a potential diagnostic tool?

Thyroid nodules are common, but only 5% of nodules are found to be malignant. In North America, the incidence of thyroid cancer is increasing. Fine needle aspirate (FNA) biopsy is the diagnostic test of choice. Unfortunately, up to 20% of FNAs are non-diagnostic. A specific molecular marker for thyr...

Full description

Saved in:
Bibliographic Details
Published in:Journal of otolaryngology 2014-07, Vol.43 (1), p.22-22
Main Authors: Brace, Matthew D, Wang, Jun, Petten, Mark, Bullock, Martin J, Makki, Fawaz, Trites, Jonathan, Taylor, S Mark, Hart, Robert D
Format: Article
Language:English
Subjects:
Aged
Analysis
Biopsy, Fine-Needle
Carcinoma - diagnosis
Carcinoma - genetics
Carcinoma - metabolism
Carcinoma, Papillary
Diagnosis, Differential
Female
Gene Expression Regulation, Neoplastic
Genetic aspects
Genetic research
Genetic transcription
Health aspects
Humans
Male
Original
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
RNA, Neoplasm - genetics
Thyroid Cancer, Papillary
Thyroid Neoplasms - diagnosis
Thyroid Neoplasms - genetics
Thyroid Neoplasms - metabolism
Thyroid Nodule - diagnosis
Transforming Growth Factor beta1 - biosynthesis
Transforming Growth Factor beta1 - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thyroid nodules are common, but only 5% of nodules are found to be malignant. In North America, the incidence of thyroid cancer is increasing. Fine needle aspirate (FNA) biopsy is the diagnostic test of choice. Unfortunately, up to 20% of FNAs are non-diagnostic. A specific molecular marker for thyroid cancer is desirable. Evidence suggests that cell signaling through transforming growth factor beta (TGF-β) is important in the development of thyroid cancer. We sought to compare the expression of TGF-β in malignant and benign thyroid nodules. From 2008-present, thyroid nodule tissue from thyroidectomy specimens was prospectively collected and stored at -80°C. RNA extraction and reverse transcription was performed on 47 samples (24 papillary thyroid cancer and 23 benign nodules). Quantitative PCR using SYBR green was performed to detect TGF-β-1 and -2. Resulting CT values were normalized against β-actin. Gene expression was calculated using the 2(-ΔC)T method. A significantly greater expression of TGF-β1 (p 
ISSN:1916-0216
1916-0208
1916-0216
DOI:10.1186/s40463-014-0022-x