Expression of microRNA‐122 contributes to apoptosis in H9C2 myocytes

The microRNAs (miRNAs) can post‐transcriptionally regulate gene expression and heart development. The Pax‐8 gene knockout mice have apparent heart abnormalities. This study investigated the role of miRNAs in regulation of cardiac apoptosis and development in the knockout mice. MicroRNA microarrays d...

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Published in:Journal of cellular and molecular medicine 2012-11, Vol.16 (11), p.2637-2646
Main Authors: Huang, Xiaoyan, Huang, Fang, Yang, Deye, Dong, Fengquan, Shi, Xiangxiang, Wang, Hongyu, Zhou, Xi, Wang, Suyun, Dai, Shengchuan
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - genetics
Caspase 3 - genetics
Caspase 3 - metabolism
Cells, Cultured
differential expression
Gene Expression Regulation, Developmental
HAND2
Heart Septal Defects - genetics
Heart Septal Defects - pathology
Heart Septum - pathology
Mice
Mice, Knockout
MicroRNAs - antagonists & inhibitors
MicroRNAs - genetics
miR‐122
Myocytes, Cardiac - pathology
Myocytes, Cardiac - physiology
Original
Paired Box Transcription Factors - genetics
PAX8 Transcription Factor
RNA, Small Interfering
Sincalide - genetics
Sincalide - metabolism
Transduction, Genetic
Up-Regulation
ventricular septum defect
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container_issue 11
container_start_page 2637
container_title Journal of cellular and molecular medicine
container_volume 16
creator Huang, Xiaoyan
Huang, Fang
Yang, Deye
Dong, Fengquan
Shi, Xiangxiang
Wang, Hongyu
Zhou, Xi
Wang, Suyun
Dai, Shengchuan
description The microRNAs (miRNAs) can post‐transcriptionally regulate gene expression and heart development. The Pax‐8 gene knockout mice have apparent heart abnormalities. This study investigated the role of miRNAs in regulation of cardiac apoptosis and development in the knockout mice. MicroRNA microarrays demonstrated differential expression of microRNAs between Pax‐8−/− and Pax‐8+/− mice, confirmed by real‐time PCR. The miR‐122 was up‐regulated by 1.92 folds in Pax‐8−/− mice. There were ventricular septum defects in Pax‐8−/− mice, and increased numbers of apoptotic cells in the left ventricular wall and interventricular septum in Pax‐8−/− mice. In H9C2 myocytes, treatment with miR‐122 mimics or miR‐122 inhibitor affects the expression of CCK‐8 and activity of Caspase‐3. The miR‐122 is up‐regulated in the myocytes of Pax‐8−/− mice and may participate in the apoptotic gene expression and pathogenesis of heart development defect.
doi_str_mv 10.1111/j.1582-4934.2012.01577.x
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The miR‐122 is up‐regulated in the myocytes of Pax‐8−/− mice and may participate in the apoptotic gene expression and pathogenesis of heart development defect.</abstract><cop>England</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>22453009</pmid><doi>10.1111/j.1582-4934.2012.01577.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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source Wiley Online Library Open Access
subjects Animals
apoptosis
Apoptosis - genetics
Caspase 3 - genetics
Caspase 3 - metabolism
Cells, Cultured
differential expression
Gene Expression Regulation, Developmental
HAND2
Heart Septal Defects - genetics
Heart Septal Defects - pathology
Heart Septum - pathology
Mice
Mice, Knockout
MicroRNAs - antagonists & inhibitors
MicroRNAs - genetics
miR‐122
Myocytes, Cardiac - pathology
Myocytes, Cardiac - physiology
Original
Paired Box Transcription Factors - genetics
PAX8 Transcription Factor
RNA, Small Interfering
Sincalide - genetics
Sincalide - metabolism
Transduction, Genetic
Up-Regulation
ventricular septum defect
title Expression of microRNA‐122 contributes to apoptosis in H9C2 myocytes
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